論文 - 詳細
| RRC ID | 38258 |
|---|---|
| 著者 | Nozaki H, Yanagida M, Koide K, Shiotani K, Kinoshita M, Kobayashi Y, Watarai S, Nakamura K, Suzuki A, Ariga T, Kushi Y. |
| タイトル | Production and characterization of monoclonal antibodies specific to lactotriaosylceramide. |
| ジャーナル | Glycobiology |
| Abstract |
We have established hybridoma cell lines producing monoclonal antibodies (mAbs) directed to N-acetylglucosaminylβ1-3galactose (GlcNAcβ1-3Gal) residue by immunizing BALB/c mice with lactotriaosylceramide (Lc(3)Cer). These obtained hybridoma cells, specific to Lc(3)Cer, were dual immunoglobulin (Ig)-producing cells which secreted both IgM and IgG molecules as antibodies. The established mAbs are able to react with not only Lc(3)Cer but also GlcNAcβ1-3-terminal glycosphingolipids (GSLs) despite branching or lactosamine chain lengths and human transferrin with terminal GlcNAc residues. Comparison of the variable regions of the cloned IgM and IgG by reversed transcription-polymerase chain reaction analysis confirmed that the variable regions determine the specificity, the other amino acids are conserved, and these mAbs are encoded by J558 and Vκ-21family genes. Furthermore, we have analyzed the expression of GSLs with GlcNAcβ1-3 epitope in acute leukemia cell lines and mouse fetal tissues using these mAbs, in which antigens were distributed comparatively. These mAbs are useful for studying the precise distribution of GlcNAcβ1-3Gal-terminating GSL expression in tissues as well as for detecting GSLs carrying terminal GlcNAcβ1-3Gal carbohydrate structure. |
| 巻・号 | 20(12) |
| ページ | 1631-42 |
| 公開日 | 2010-12-1 |
| DOI | 10.1093/glycob/cwq117 |
| PII | cwq117 |
| PMID | 20693232 |
| MeSH | Animals Antibodies, Monoclonal, Murine-Derived / biosynthesis Antibodies, Monoclonal, Murine-Derived / genetics Antibodies, Monoclonal, Murine-Derived / immunology* Antibody Specificity / genetics Antibody Specificity / immunology* Female HL-60 Cells Humans Immunoglobulin G / biosynthesis Immunoglobulin G / genetics Immunoglobulin G / immunology* Immunoglobulin M / biosynthesis Immunoglobulin M / genetics Immunoglobulin M / immunology* Immunoglobulin Variable Region / biosynthesis Immunoglobulin Variable Region / genetics Immunoglobulin Variable Region / immunology* K562 Cells Lactosylceramides / biosynthesis Lactosylceramides / genetics Lactosylceramides / immunology* Mice Mice, Inbred BALB C U937 Cells |
| IF | 4.06 |
| 引用数 | 7 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) K562(RCB0027) U-937 DE-4(RCB0435) |