| RRC ID |
38290
|
| 著者 |
Norikura T, Mukai Y, Fujita S, Mikame K, Funaoka M, Sato S.
|
| タイトル |
Lignophenols decrease oleate-induced apolipoprotein-B secretion in HepG2 cells.
|
| ジャーナル |
Basic Clin Pharmacol Toxicol
|
| Abstract |
Lignin is one of the components in the plant cell wall, after cellulose, making up 20-30% of the global plant biomass. Lignophenols (LPs) are derivatives of lignin with high phenolic function and antioxidant properties. However, their medicinal property is not well characterised. Apolipoprotein-B (apo-B) is an essential component in very low-density lipoprotein, and high levels of serum apolipoprotein-B (apo-B) are a major factor for coronary heart disease. In this study, we examined the effect of lignophenols on apo-B secretion in HepG2 cells. HepG2 cells were treated with varying concentrations of LPs and 0.8 mm sodium oleate. LPs decreased oleate-induced apo-B secretion in a dose-dependent manner. LPs also decreased oleate-induced microsomal triglyceride transfer protein (MTTP) mRNA expression and cellular total cholesterol, suggesting that lipid bioavailability and lipidation of lipoprotein are likely involved in the decreased secretion of apo-B. Furthermore, LPs decreased oleate-induced mature sterol regulatory element binding protein 2 (SREBP-2), a transcription factor that activates cholesterol biosynthesis. This is the first study to show that LPs can decrease oleate-induced apo-B secretion in HepG2 cells. The modulations of MTTP mRNA expression, cellular total cholesterol metabolism and mature SREBP-2 expression may be important factors in the regulation of apo-B secretion by LPs.
|
| 巻・号 |
107(4)
|
| ページ |
813-7
|
| 公開日 |
2010-10-1
|
| DOI |
10.1111/j.1742-7843.2010.00575.x
|
| PII |
PTO575
|
| PMID |
20412465
|
| MeSH |
Apolipoproteins B / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism
Cholesterol / metabolism
Dose-Response Relationship, Drug
Hep G2 Cells
Humans
Lignin / pharmacology*
Oleic Acid / pharmacology*
Phenols / pharmacology*
RNA, Messenger / metabolism
Sterol Regulatory Element Binding Protein 2 / genetics
Sterol Regulatory Element Binding Protein 2 / metabolism
|
| IF |
2.651
|
| 引用数 |
12
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
TOXICOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Hep G2(RCB1648) |
