RRC ID 38291
Author Akutsu N, Yamamoto H, Sasaki S, Taniguchi H, Arimura Y, Imai K, Shinomura Y.
Title Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma.
Journal World J. Gastroenterol.
Abstract AIM:To clarify the association of glypican-3 (GPC3) expression with Wnt and other growth signaling molecules in hepatocellular carcinoma (HCC).
METHODS:Expression of GPC3, Wnt, matrix metalloproteinases (MMPs), sulfatase (SULF)1, SULF2, and other growth signaling molecules was analyzed in HCC cell lines and tissue samples by real-time reverse transcription-polymerase chain reaction, immunoblotting, and/or immunostaining. Expression of various genes in GPC3 siRNA-transfected HCC cells was analyzed.
RESULTS:GPC3 was overexpressed in most HCCs at mRNA and protein levels and its serum levels were significantly higher in patients with HCC than in non-HCC subjects (P < 0.05). Altered expressions of various MMPs and growth signaling molecules, some of which were correlated with GPC3 expression, were observed in HCCs. Down-regulation of GPC3 expression by siRNA in GPC3-overexpressing HCC cell lines resulted in a significant decrease in expressions of MMP2, MMP14, fibroblast growth factor receptor 1, insulin-like growth factor 1 receptor. GPC3 expression was significantly correlated with nuclear/cytoplasmic localization of beta-catenin.
CONCLUSION:These results suggest that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC.
Volume 16(28)
Pages 3521-8
Published 2010-7-28
DOI 10.3748/wjg.v16.i28.3521
PMID 20653060
PMC PMC2909551
MeSH Carcinoma, Hepatocellular / metabolism* Cell Line, Tumor Glypicans / metabolism* Humans Intercellular Signaling Peptides and Proteins / metabolism* Liver Neoplasms / metabolism* Matrix Metalloproteinases / metabolism Sulfotransferases / metabolism Wnt Proteins / metabolism
IF 3.411
Times Cited 15
Human and Animal Cells