論文 - 詳細
RRC ID | 38291 |
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著者 | Akutsu N, Yamamoto H, Sasaki S, Taniguchi H, Arimura Y, Imai K, Shinomura Y. |
タイトル | Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma. |
ジャーナル | World J Gastroenterol |
Abstract |
AIM:To clarify the association of glypican-3 (GPC3) expression with Wnt and other growth signaling molecules in hepatocellular carcinoma (HCC). METHODS:Expression of GPC3, Wnt, matrix metalloproteinases (MMPs), sulfatase (SULF)1, SULF2, and other growth signaling molecules was analyzed in HCC cell lines and tissue samples by real-time reverse transcription-polymerase chain reaction, immunoblotting, and/or immunostaining. Expression of various genes in GPC3 siRNA-transfected HCC cells was analyzed. RESULTS:GPC3 was overexpressed in most HCCs at mRNA and protein levels and its serum levels were significantly higher in patients with HCC than in non-HCC subjects (P < 0.05). Altered expressions of various MMPs and growth signaling molecules, some of which were correlated with GPC3 expression, were observed in HCCs. Down-regulation of GPC3 expression by siRNA in GPC3-overexpressing HCC cell lines resulted in a significant decrease in expressions of MMP2, MMP14, fibroblast growth factor receptor 1, insulin-like growth factor 1 receptor. GPC3 expression was significantly correlated with nuclear/cytoplasmic localization of beta-catenin. CONCLUSION:These results suggest that GPC3, in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC. |
巻・号 | 16(28) |
ページ | 3521-8 |
公開日 | 2010-7-28 |
DOI | 10.3748/wjg.v16.i28.3521 |
PMID | 20653060 |
PMC | PMC2909551 |
MeSH | Carcinoma, Hepatocellular / metabolism* Cell Line, Tumor Glypicans / metabolism* Humans Intercellular Signaling Peptides and Proteins / metabolism* Liver Neoplasms / metabolism* Matrix Metalloproteinases / metabolism Sulfatases Sulfotransferases / metabolism Wnt Proteins / metabolism |
IF | 3.665 |
引用数 | 23 |
WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
リソース情報 | |
ヒト・動物細胞 | Li-7(RCB1941) |