RRC ID |
38298
|
著者 |
Nakazora S, Matsumine A, Iino T, Hasegawa M, Kinoshita A, Uemura K, Niimi R, Uchida A, Sudo A.
|
タイトル |
The cleavage of N-cadherin is essential for chondrocyte differentiation.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
The aggregation of chondroprogenitor mesenchymal cells into precartilage condensation represents one of the earliest events in chondrogenesis. N-cadherin is a key cell adhesion molecule implicated in chondrogenic differentiation. Recently, ADAM10-mediated cleavage of N-cadherin has been reported to play an important role in cell adhesion, migration, development and signaling. However, the significance of N-cadherin cleavage in chondrocyte differentiation has not been determined. In the present study, we found that the protein turnover of N-cadherin is accelerated during the early phase of chondrogenic differentiation in ATDC5 cells. Therefore, we generated the subclones of ATDC5 cells overexpressing wild-type N-cadherin, and two types of subclones overexpressing a cleavage-defective N-cadherin mutant, and examined the response of these cells to insulin stimulation. The ATDC5 cells overexpressing cleavage-defective mutants severely prevented the formation of cartilage aggregates, proteoglycan production and the induction of chondrocyte marker gene expression, such as type II collagen, aggrecan and type X collagen. These results suggested that the cleavage of N-cadherin is essential for chondrocyte differentiation.
|
巻・号 |
400(4)
|
ページ |
493-9
|
公開日 |
2010-10-1
|
DOI |
10.1016/j.bbrc.2010.08.070
|
PII |
S0006-291X(10)01565-2
|
PMID |
20735983
|
MeSH |
ADAM Proteins / metabolism
ADAM10 Protein
Amino Acid Sequence
Amyloid Precursor Protein Secretases / metabolism
Animals
Antigens, CD / genetics
Antigens, CD / metabolism*
Cadherins / genetics
Cadherins / metabolism*
Cartilage / cytology
Cartilage / growth & development*
Cartilage / metabolism
Cell Differentiation*
Cell Line, Tumor
Chondrocytes / cytology*
Chondrocytes / metabolism
Chondrogenesis*
Humans
Membrane Proteins / metabolism
Mice
Molecular Sequence Data
Mutation
Proteoglycans / metabolism
|
IF |
2.985
|
引用数 |
12
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|