論文 - 詳細
| RRC ID | 38298 |
|---|---|
| 著者 | Nakazora S, Matsumine A, Iino T, Hasegawa M, Kinoshita A, Uemura K, Niimi R, Uchida A, Sudo A. |
| タイトル | The cleavage of N-cadherin is essential for chondrocyte differentiation. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The aggregation of chondroprogenitor mesenchymal cells into precartilage condensation represents one of the earliest events in chondrogenesis. N-cadherin is a key cell adhesion molecule implicated in chondrogenic differentiation. Recently, ADAM10-mediated cleavage of N-cadherin has been reported to play an important role in cell adhesion, migration, development and signaling. However, the significance of N-cadherin cleavage in chondrocyte differentiation has not been determined. In the present study, we found that the protein turnover of N-cadherin is accelerated during the early phase of chondrogenic differentiation in ATDC5 cells. Therefore, we generated the subclones of ATDC5 cells overexpressing wild-type N-cadherin, and two types of subclones overexpressing a cleavage-defective N-cadherin mutant, and examined the response of these cells to insulin stimulation. The ATDC5 cells overexpressing cleavage-defective mutants severely prevented the formation of cartilage aggregates, proteoglycan production and the induction of chondrocyte marker gene expression, such as type II collagen, aggrecan and type X collagen. These results suggested that the cleavage of N-cadherin is essential for chondrocyte differentiation. |
| 巻・号 | 400(4) |
| ページ | 493-9 |
| 公開日 | 2010-10-1 |
| DOI | 10.1016/j.bbrc.2010.08.070 |
| PII | S0006-291X(10)01565-2 |
| PMID | 20735983 |
| MeSH | ADAM Proteins / metabolism ADAM10 Protein Amino Acid Sequence Amyloid Precursor Protein Secretases / metabolism Animals Antigens, CD / genetics Antigens, CD / metabolism* Cadherins / genetics Cadherins / metabolism* Cartilage / cytology Cartilage / growth & development* Cartilage / metabolism Cell Differentiation* Cell Line, Tumor Chondrocytes / cytology* Chondrocytes / metabolism Chondrogenesis* Humans Membrane Proteins / metabolism Mice Molecular Sequence Data Mutation Proteoglycans / metabolism |
| IF | 2.985 |
| 引用数 | 12 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |