RRC ID 38298
著者 Nakazora S, Matsumine A, Iino T, Hasegawa M, Kinoshita A, Uemura K, Niimi R, Uchida A, Sudo A.
タイトル The cleavage of N-cadherin is essential for chondrocyte differentiation.
ジャーナル Biochem Biophys Res Commun
Abstract The aggregation of chondroprogenitor mesenchymal cells into precartilage condensation represents one of the earliest events in chondrogenesis. N-cadherin is a key cell adhesion molecule implicated in chondrogenic differentiation. Recently, ADAM10-mediated cleavage of N-cadherin has been reported to play an important role in cell adhesion, migration, development and signaling. However, the significance of N-cadherin cleavage in chondrocyte differentiation has not been determined. In the present study, we found that the protein turnover of N-cadherin is accelerated during the early phase of chondrogenic differentiation in ATDC5 cells. Therefore, we generated the subclones of ATDC5 cells overexpressing wild-type N-cadherin, and two types of subclones overexpressing a cleavage-defective N-cadherin mutant, and examined the response of these cells to insulin stimulation. The ATDC5 cells overexpressing cleavage-defective mutants severely prevented the formation of cartilage aggregates, proteoglycan production and the induction of chondrocyte marker gene expression, such as type II collagen, aggrecan and type X collagen. These results suggested that the cleavage of N-cadherin is essential for chondrocyte differentiation.
巻・号 400(4)
ページ 493-9
公開日 2010-10-1
DOI 10.1016/j.bbrc.2010.08.070
PII S0006-291X(10)01565-2
PMID 20735983
MeSH ADAM Proteins / metabolism ADAM10 Protein Amino Acid Sequence Amyloid Precursor Protein Secretases / metabolism Animals Antigens, CD / genetics Antigens, CD / metabolism* Cadherins / genetics Cadherins / metabolism* Cartilage / cytology Cartilage / growth & development* Cartilage / metabolism Cell Differentiation* Cell Line, Tumor Chondrocytes / cytology* Chondrocytes / metabolism Chondrogenesis* Humans Membrane Proteins / metabolism Mice Molecular Sequence Data Mutation Proteoglycans / metabolism
IF 2.985
引用数 12
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞