論文 - 詳細
| RRC ID | 38300 |
|---|---|
| 著者 | Tamura K, Mizutani T, Haga H, Kawabata K. |
| タイトル | Nano-mechanical properties of living cells expressing constitutively active RhoA effectors. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Filamentous actin and myosin-II are major determinants of cell mechanics and are tightly regulated by a small guanosine triphosphatase, RhoA, and its downstream effectors. We examined the effects of constitutively active mutants of RhoA effectors, which have not been reported before, on cortical stiffness of living cells by using scanning probe microscopy, fluorescence microscopy, and truncated mutants of RhoA effectors labeled with a fluorescent protein. Our data indicated that expression of a constitutively active mutant of Dia1, a formin-family actin polymerizer, enhanced cortical stiffness and increased actin filament quantity in cells. Furthermore, expression of a constitutively active mutant of Rho-associated coiled-coil kinase, a myosin-II activator, softened the cell cortex but increased myosin-II activity. Our findings provide new insights into anomalous mechanics of cells, which is a topic of current interest in a variety of biological research fields. |
| 巻・号 | 403(3-4) |
| ページ | 363-7 |
| 公開日 | 2010-12-17 |
| DOI | 10.1016/j.bbrc.2010.11.036 |
| PII | S0006-291X(10)02094-2 |
| PMID | 21078298 |
| MeSH | Animals Carrier Proteins / genetics Formins Mice Microscopy, Fluorescence Mutation NIH 3T3 Cells Nanostructures Stress Fibers / physiology Stress, Mechanical* rho GTP-Binding Proteins / biosynthesis* rho GTP-Binding Proteins / genetics rhoA GTP-Binding Protein |
| IF | 2.985 |
| 引用数 | 6 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |