論文 - 詳細
| RRC ID | 38310 |
|---|---|
| 著者 | Wu YX, Fang X. |
| タイトル | Apigenin, chrysin, and luteolin selectively inhibit chymotrypsin-like and trypsin-like proteasome catalytic activities in tumor cells. |
| ジャーナル | Planta Med |
| Abstract |
The ubiquitin-proteasome pathway has an important role in regulating apoptosis and the cell cycle. The function of proteasomes is mediated by three main catalytic activities: (1) chymotrypsin-like (CT-L), (2) trypsin-like (T-L), and (3) peptidylglutamyl peptide hydrolyzing (PGPH). Recently, proteasome inhibitors have been revealed to have an antitumor effect, and have been used to treat cancers such as multiple myeloma. Previous studies have reported that some flavonoids can inhibit proteasome activity in tumor cells. To further investigate the proteasome-inhibitory mechanism of flavonoids, we examined the effects of the plant flavonoids apigenin, chrysin, and luteolin on the three individual catalytic activities in various cancer cell lines. Using fluorogenic substrates specific for proteasome catalytic subunits, we demonstrated the subunit specificity of each flavonoid. Addition of apigenin, chrysin and luteolin inhibited CT-L and T-L catalytic activities in a dose-dependent manner, whereas their effect on PGPH catalytic activity was weak. Our study suggested that these flavonoids have a specific role in inhibition of CT-L and T-L proteasome catalytic activities. |
| 巻・号 | 76(2) |
| ページ | 128-32 |
| 公開日 | 2010-2-1 |
| DOI | 10.1055/s-0029-1186004 |
| PMID | 19653143 |
| MeSH | Antineoplastic Agents, Phytogenic / pharmacology* Antineoplastic Agents, Phytogenic / therapeutic use Apigenin / pharmacology Apigenin / therapeutic use Cell Line, Tumor Chymotrypsin / metabolism* Dose-Response Relationship, Drug Flavonoids / pharmacology* Flavonoids / therapeutic use Humans Hydrolysis Luteolin / pharmacology Luteolin / therapeutic use Neoplasms / drug therapy Neoplasms / metabolism* Peptides / metabolism Plant Extracts / pharmacology* Plant Extracts / therapeutic use Proteasome Endopeptidase Complex / metabolism* Trypsin / metabolism* Ubiquitin |
| IF | 2.687 |
| 引用数 | 23 |
| WOS 分野 | INTEGRATIVE & COMPLEMENTARY MEDICINE PHARMACOLOGY & PHARMACY PLANT SCIENCES CHEMISTRY, MEDICINAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | |