Reference - Detail
| RRC ID | 38321 |
|---|---|
| Author | Kawano M, Thet MM, Makino T, Kushida T, Sakagami H. |
| Title | DNA microarray analysis of signaling pathway in macrophages stimulated by lignin-carbohydrate complex from lentinus edodes mycelia (LEM) extract. |
| Journal | Anticancer Res |
| Abstract |
In order to investigate the action point of lignin-carbohydrate complex (Fr4) from Lentinus edodes mycelia extract, DNA microarray analysis was performed, using mouse macrophage-like J774.1 cells. Among seven lignin-carbohydrate complex fractions, Fr4 showed the highest stimulatory activity of tumor necrosis factor production by mouse macrophage-like J774.1 cells, as well as its previously reported anti-HIV activity. Fr4 is composed of lignin precursors such as vanillic acid, syringic acid, p-coumaric acid and ferulic acid, with trace amounts of flavonoids and tannins, and negligible amount of lipopolysaccharides (LPS), confirming the authenticity of Fr4 as a lignin. DNA microarray analysis suggested that Fr4 may affect immune response-related gene expression; however, it may not affect the expression of as many genes as LPS does. |
| Volume | 30(7) |
| Pages | 2567-76 |
| Published | 2010-7-1 |
| PII | 30/7/2567 |
| PMID | 20682984 |
| MeSH | Animals Cell Line Cluster Analysis Gene Expression / drug effects Lignin / analysis Lignin / isolation & purification Lignin / pharmacology* Lipopolysaccharides / analysis Lipopolysaccharides / pharmacology Macrophages / drug effects* Macrophages / immunology Macrophages / metabolism Mice Oligonucleotide Array Sequence Analysis / methods Principal Component Analysis Shiitake Mushrooms / chemistry* Signal Transduction / drug effects Stimulation, Chemical Tumor Necrosis Factor-alpha / biosynthesis |
| IF | 1.994 |
| Times Cited | 11 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | J774.1 |