論文 - 詳細
| RRC ID | 38335 |
|---|---|
| 著者 | Shibata H, Inuzuka T, Yoshida H, Sugiura H, Wada I, Maki M. |
| タイトル | The ALG-2 binding site in Sec31A influences the retention kinetics of Sec31A at the endoplasmic reticulum exit sites as revealed by live-cell time-lapse imaging. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
ALG-2, a member of the penta-EF-hand protein family, interacts Ca²+-dependently with a COPII component, Sec31A. In this study, we first established HeLa cells stably expressing green fluorescent protein-fused ALG-2 (GFP-ALG-2) and red fluorescent protein-fused Sec31A (Sec31A-RFP). After inducing Ca²+-mobilization, the cytoplasmic distribution of GFP-ALG-2 changed from a diffuse to a punctate pattern, which extensively overlapped with the Sec31A-RFP-positive structures, indicating that ALG-2 is recruited to the endoplasmic reticulum exit sites (ERES) in living cells. Next, overlay experiments with biotin-labeled ALG-2 were done to dissect the ALG-2 binding site (ABS). They revealed that a sequence comprising amino acid residues 839-851 in the Pro-rich region was necessary and sufficient for direct binding to ALG-2. Finally, fluorescence recovery after photobleaching analysis indicated that the ABS deletion reduced the high-affinity population of Sec31A to the ERES, suggesting that the ABS is one of the key determinants of the retention kinetics of Sec31A at ERES. |
| 巻・号 | 74(9) |
| ページ | 1819-26 |
| 公開日 | 2010-1-1 |
| DOI | 10.1271/bbb.100215 |
| PII | JST.JSTAGE/bbb/100215 |
| PMID | 20834162 |
| MeSH | Apoptosis Regulatory Proteins / metabolism* Binding Sites COP-Coated Vesicles Calcium-Binding Proteins / metabolism* Endoplasmic Reticulum / metabolism* HeLa Cells Humans Kinetics Luminescent Proteins Protein Binding Protein Transport Recombinant Fusion Proteins Time-Lapse Imaging / methods* Vesicular Transport Proteins / metabolism* |
| IF | 1.516 |
| 引用数 | 28 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |