論文 - 詳細
| RRC ID | 38341 |
|---|---|
| 著者 | Miyazaki T, Miyauchi S, Tawada A, Anada T, Suzuki O. |
| タイトル | Effect of chondroitin sulfate-E on the osteoclastic differentiation of RAW264 cells. |
| ジャーナル | Dent Mater J |
| Abstract |
The present study was designed to investigate whether chondroitin sulfate (CS)-E, a CS structural isomer variant, alter the differentiation of macrophage cell line RAW264 cells to osteoclast-like cells. CS-B, CS-E, low molecular weight CS-E, synthetic chondroitin polysulfate (CPS) and heparin significantly inhibited the formation of tartrate-resistant acid phosphatase-positive multinuclear cells and pit formation on calcium phosphate (CaP)-coated plates. CS-E pre-coated on the CaP plate also inhibited pit formation. Digestion of CS on the cell surface by chondroitinase showed no effect on the osteoclastic differentiation of RAW264 cells whereas inhibitory effect on the differentiation of osteoblastic cell line MC3T3-E1. On the other hand, exogenously added fluorescein-labeled CS-E directory bound to fibronectin and RAW264 cells. These results suggest that CS-E structure on the surface of osteoblasts or bone matrix binds to cell adhesion molecule such as integrin on the pre-osteoclastic cells and inhibits the differentiation into osteoclasts. CS-E may have a potential in treating bone defect if combined with CaP materials. |
| 巻・号 | 29(4) |
| ページ | 403-10 |
| 公開日 | 2010-8-1 |
| DOI | 10.4012/dmj.2009-132 |
| PII | JST.JSTAGE/dmj/2009-132 |
| PMID | 20610874 |
| MeSH | 3T3 Cells Acid Phosphatase / antagonists & inhibitors Animals Bone Matrix / metabolism Bone Resorption / metabolism Calcium Phosphates / analysis Calcium Phosphates / metabolism Cell Culture Techniques Cell Differentiation / drug effects Cell Line Chondroitin ABC Lyase / pharmacology Chondroitin Sulfates / pharmacology* Dermatan Sulfate Durapatite / analysis Fibronectins / metabolism Fluorescein Fluorescent Dyes Glycosaminoglycans / metabolism Heparin / pharmacology Integrins / metabolism Isoenzymes / antagonists & inhibitors Isomerism Macrophages / drug effects* Mice Molecular Weight Osteoblasts / drug effects Osteoclasts / drug effects* Osteoclasts / physiology Protein Binding / drug effects RANK Ligand / pharmacology Spectroscopy, Fourier Transform Infrared Tartrate-Resistant Acid Phosphatase |
| IF | 1.359 |
| 引用数 | 18 |
| WOS 分野 | DENTISTRY, ORAL SURGERY & MEDICINE MATERIALS SCIENCE, BIOMATERIALS |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |