論文 - 詳細
| RRC ID | 38344 |
|---|---|
| 著者 | Ko JA, Yanai R, Chikama T, Nishida T. |
| タイトル | Downregulation of matrix metalloproteinase-2 in corneal fibroblasts by interleukin-1 receptor antagonist released from corneal epithelial cells. |
| ジャーナル | Invest Ophthalmol Vis Sci |
| Abstract |
PURPOSE:The authors previously showed that the expression of various junctional proteins in corneal epithelial cells or corneal fibroblasts (CFs) is regulated by the presence of the other cell type in a coculture system. In this study, the effect of corneal epithelial cells on the expression of matrix metalloproteinases (MMPs) in CFs was studied. METHODS:Human CFs and simian virus 40-transformed human corneal epithelial (HCE) cells were cultured on opposite sides of a collagen vitrigel membrane. Expression of MMPs in CFs was examined by reverse transcription-polymerase chain reaction and immunoblot analyses. RESULTS:The amounts of MMP-2 mRNA and protein in CFs were decreased by the presence of HCE cells. HCE cells had no effect on the expression of MMP-1 in CFs. HCE cells released interleukin (IL)-1 receptor antagonist (IL-1RA) into the culture medium, and depletion of IL-1RA in HCE cells by RNA interference largely abolished the effect of these cells on MMP-2 expression in CFs. The downregulation of MMP-2 expression in CFs by HCE cells was blocked by an inhibitor of signaling by the mitogen-activated protein kinase (MAPK) ERK (PD98059) but was not affected by those of signaling by the MAPKs p38 (SB203580) or JNK (SP600125). CONCLUSIONS:Corneal epithelial cells downregulated expression of MMP-2 in CFs in a manner dependent at least in part on the release of IL-1RA from the former cells. This effect might contribute to the attenuation of corneal stromal remodeling by corneal epithelial cells. |
| 巻・号 | 51(12) |
| ページ | 6286-93 |
| 公開日 | 2010-12-1 |
| DOI | 10.1167/iovs.09-4753 |
| PII | iovs.09-4753 |
| PMID | 20688742 |
| MeSH | Cell Line Cell Line, Transformed Coculture Techniques Cornea / cytology Cornea / enzymology* Down-Regulation* Epithelium, Corneal / cytology Epithelium, Corneal / metabolism* Fibroblasts / cytology Fibroblasts / enzymology* Humans Immunoblotting Interleukin 1 Receptor Antagonist Protein / genetics Interleukin 1 Receptor Antagonist Protein / metabolism* Matrix Metalloproteinase 2 / genetics* Matrix Metalloproteinase 2 / metabolism* Mitogen-Activated Protein Kinase Kinases / physiology RNA, Messenger / genetics RNA, Small Interfering / genetics Receptors, Interleukin-1 / antagonists & inhibitors Reverse Transcriptase Polymerase Chain Reaction Transfection |
| IF | 3.47 |
| 引用数 | 8 |
| WOS 分野 | OPHTHALMOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |