RRC ID 38352
著者 Dui W, Lu W, Ma J, Jiao R.
タイトル A systematic phenotypic screen of F-box genes through a tissue-specific RNAi-based approach in Drosophila.
ジャーナル J Genet Genomics
Abstract F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 out of 75 F-box proteins have experimentally documented substrates, whereas in Drosophila 12 F-box proteins have been characterized with known substrates. To systematically investigate the genetic and molecular functions of F-box proteins in Drosophila, we performed a survey of the literature and databases. We identified 45 Drosophila genes that encode proteins containing at least one F-box domain. We collected publically available RNAi lines against these genes and used them in a tissue-specific RNAi-based phenotypic screen. Here, we present our systematic phenotypic dataset from the eye, the wing and the notum. This dataset is the first of its kind and represents a useful resource for future studies of the molecular and genetic functions of F-box genes in Drosophila. Our results show that, as expected, F-box genes in Drosophila have regulatory roles in a diverse array of processes including cell proliferation, cell growth, signal transduction, and cellular and animal survival.
巻・号 39(8)
ページ 397-413
公開日 2012-8-20
DOI 10.1016/j.jgg.2012.05.009
PII S1673-8527(12)00116-6
PMID 22884096
MeSH Animals Drosophila / genetics* Drosophila / growth & development Drosophila / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Eye / growth & development Eye / metabolism F-Box Proteins / genetics* F-Box Proteins / metabolism Molecular Sequence Data Organ Specificity Phenotype RNA Interference* Wings, Animal / growth & development Wings, Animal / metabolism
IF 5.065
引用数 11
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ 3412R-1 4063R-2 4063R-4 32085R-2 2010R-1 2010R-2 10800R-2 15010R-2 15010R-3 9952R-1 9952R-2 3428R-1 3428R-3 4911R-1 5003R-1 5003R-3 15437R-1 15437R-2 8272R-1 8272R-2 31633R-2