論文 - 詳細
| RRC ID | 38352 |
|---|---|
| 著者 | Dui W, Lu W, Ma J, Jiao R. |
| タイトル | A systematic phenotypic screen of F-box genes through a tissue-specific RNAi-based approach in Drosophila. |
| ジャーナル | J Genet Genomics |
| Abstract |
F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 out of 75 F-box proteins have experimentally documented substrates, whereas in Drosophila 12 F-box proteins have been characterized with known substrates. To systematically investigate the genetic and molecular functions of F-box proteins in Drosophila, we performed a survey of the literature and databases. We identified 45 Drosophila genes that encode proteins containing at least one F-box domain. We collected publically available RNAi lines against these genes and used them in a tissue-specific RNAi-based phenotypic screen. Here, we present our systematic phenotypic dataset from the eye, the wing and the notum. This dataset is the first of its kind and represents a useful resource for future studies of the molecular and genetic functions of F-box genes in Drosophila. Our results show that, as expected, F-box genes in Drosophila have regulatory roles in a diverse array of processes including cell proliferation, cell growth, signal transduction, and cellular and animal survival. |
| 巻・号 | 39(8) |
| ページ | 397-413 |
| 公開日 | 2012-8-20 |
| DOI | 10.1016/j.jgg.2012.05.009 |
| PII | S1673-8527(12)00116-6 |
| PMID | 22884096 |
| MeSH | Animals Drosophila / genetics* Drosophila / growth & development Drosophila / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Eye / growth & development Eye / metabolism F-Box Proteins / genetics* F-Box Proteins / metabolism Molecular Sequence Data Organ Specificity Phenotype RNA Interference* Wings, Animal / growth & development Wings, Animal / metabolism |
| IF | 5.065 |
| 引用数 | 11 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 3412R-1 4063R-2 4063R-4 32085R-2 2010R-1 2010R-2 10800R-2 15010R-2 15010R-3 9952R-1 9952R-2 3428R-1 3428R-3 4911R-1 5003R-1 5003R-3 15437R-1 15437R-2 8272R-1 8272R-2 31633R-2 |