RRC ID 38359
Author Kawakubo K, Ohnishi S, Hatanaka Y, Hatanaka KC, Hosono H, Kubota Y, Kamiya M, Kuwatani M, Kawakami H, Urano Y, Sakamoto N.
Title Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study.
Journal Mol Imaging Biol
Abstract PURPOSE:Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT).
PROCEDURES:We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min.
RESULTS:GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05).
CONCLUSIONS:Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
Volume 18(3)
Pages 463-71
Published 2016-6
DOI 10.1007/s11307-015-0898-5
PII 10.1007/s11307-015-0898-5
PMID 26431952
MeSH Aged Animals Cell Line, Tumor Endoscopic Ultrasound-Guided Fine Needle Aspiration / methods* Feasibility Studies Female Fluorescent Dyes / metabolism* Humans Male Microscopy, Fluorescence Middle Aged Pancreas / pathology* Pancreatic Neoplasms / enzymology Pancreatic Neoplasms / pathology Pilot Projects Rats Signal Processing, Computer-Assisted gamma-Glutamyltransferase / metabolism*
IF 3.341
Times Cited 2
Human and Animal Cells KP4(RCB1005)