Reference - Detail
|Author||Kawakubo K, Ohnishi S, Hatanaka Y, Hatanaka KC, Hosono H, Kubota Y, Kamiya M, Kuwatani M, Kawakami H, Urano Y, Sakamoto N.|
|Title||Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration: a Pilot Study.|
|Journal||Mol Imaging Biol|
PURPOSE:Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT).
PROCEDURES:We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min.
RESULTS:GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05).
CONCLUSIONS:Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
|MeSH||Aged Animals Cell Line, Tumor Endoscopic Ultrasound-Guided Fine Needle Aspiration / methods* Feasibility Studies Female Fluorescent Dyes / metabolism* Humans Male Microscopy, Fluorescence Middle Aged Pancreas / pathology* Pancreatic Neoplasms / enzymology Pancreatic Neoplasms / pathology Pilot Projects Rats Signal Processing, Computer-Assisted gamma-Glutamyltransferase / metabolism*|
|WOS Category||RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING|
|Human and Animal Cells||KP4(RCB1005)|