RRC ID 38366
著者 Usui M, Sato T, Yamamoto G, Okamatsu Y, Hanatani T, Moritani Y, Sano K, Yamamoto M, Nakashima K.
タイトル Gingival epithelial cells support osteoclastogenesis by producing receptor activator of nuclear factor kappa B ligand via protein kinase A signaling.
ジャーナル J Periodontal Res
Abstract BACKGROUND AND OBJECTIVE:Periodontal disease is dental plaque-induced inflammatory disease of the periodontal tissues that results in bone loss in the affected teeth. During bone resorption, receptor activator of nuclear factor kappa B ligand (RANKL) is an essential factor that regulates osteoclastogenesis. Recently, we found that gingival epithelial cells (GECs) in periodontal tissue produce RANKL, the expression of which is regulated by tumor necrosis factor-α and protein kinase A signaling. In this study, we asked whether RANKL-producing GECs induce bone marrow macrophages (BMMs) to form osteoclasts in a co-culture system.
MATERIAL AND METHODS:Ca9-22 GECs and osteoclast precursor BMMs were co-cultured with or without the protein kinase A signaling activator forskolin or inhibitor H89 to examine whether the RANKL-producing GECs could be induced to form osteoclasts, as determined using a pit formation assay.
RESULTS:Osteoclasts formed spontaneously in co-cultures of Ca9-22 cells and BMMs, even in the absence of RANKL. The cells were cultured on bone slices for 14 d, at which time resorption pits were observed. Forskolin treatment significantly increased osteoclast numbers in these co-cultures, but forskolin alone did not induce osteoclast formation by BMMs.
CONCLUSION:GECs producing RANKL are able to support osteoclastogenesis in an in vitro co-culture system using GECs and BMMs, in a process promoted by forskolin.
巻・号 51(4)
ページ 462-70
公開日 2016-8-1
DOI 10.1111/jre.12323
PMID 26432443
MeSH Bone Marrow Cells / metabolism* Cells, Cultured Coculture Techniques Cyclic AMP-Dependent Protein Kinases / metabolism Epithelial Cells / metabolism* Gingiva / cytology* Gingiva / metabolism Humans Macrophages / metabolism* Osteoclasts / physiology* Osteogenesis / physiology* RANK Ligand / biosynthesis*
IF 2.926
引用数 4
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE
リソース情報
ヒト・動物細胞 Ca9-22(RCB1976)