論文 - 詳細
| RRC ID | 38395 |
|---|---|
| 著者 | Yoshizaki H, Kuwajima Y, Minato H, Kiyokawa E. |
| タイトル | Regulation of Ripply1 expression in MDCK organoids. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Epithelial organs are made of a well-polarized monolayer of epithelial cells, and their morphology must be maintained for their proper function. To examine the genes that are specifically expressed in the late stages of cystogenesis and are involved in maintaining the morphology of the mature cysts, we performed a microarray analysis comparing the mRNA expression between the early and late stages of Madin-Darby Canine Kidney (MDCK) cystogenesis. We found that one of the gene candidates, Ripply1, was expressed higher in the late stages, and its expression was also transiently much higher in the early stages. Although the protein expression showed similar kinetics, depletion of Ripply1 had only a slight effect on organoid growth. Unexpectedly, we found that the Ripply1 protein is degraded by the proteasome system. Mutant analysis suggests that Ripply1 is not ubiquitinated directly, but rather is degraded only after binding to Transducin-like Enhancer of Split (TLE)1, a transcriptional repressor. Ripply1 is degraded in the nucleus, and this degradation is inhibited during the mitosis. These data indicate for the first time that Ripply1 expression is regulated at the protein level. |
| 巻・号 | 468(1-2) |
| ページ | 337-42 |
| 公開日 | 2015-12-4 |
| DOI | 10.1016/j.bbrc.2015.10.099 |
| PII | S0006-291X(15)30799-3 |
| PMID | 26514726 |
| MeSH | Animals Dogs Gene Expression Regulation Madin Darby Canine Kidney Cells / cytology* Madin Darby Canine Kidney Cells / metabolism Mitosis Nuclear Proteins / genetics* Nuclear Proteins / metabolism Organoids / cytology* Organoids / metabolism Proteolysis RNA Interference RNA, Small Interfering / genetics Repressor Proteins / genetics* Repressor Proteins / metabolism |
| IF | 2.985 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MDCK(RCB0995) |