RRC ID 38406
著者 Abo M, Urano Y, Hanaoka K, Terai T, Komatsu T, Nagano T.
タイトル Development of a highly sensitive fluorescence probe for hydrogen peroxide.
ジャーナル J Am Chem Soc
Abstract Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.
巻・号 133(27)
ページ 10629-37
公開日 2011-7-13
DOI 10.1021/ja203521e
PMID 21692459
MeSH Animals Cell Line, Tumor Electron Transport Fluoresceins / analysis* Fluoresceins / chemistry* Fluorescence Fluorescent Dyes / chemistry* Humans Hydrogen Peroxide / analysis* Macrophages / chemistry Mice
IF 14.612
引用数 230
WOS 分野 CHEMISTRY, MULTIDISCIPLINARY
リソース情報
ヒト・動物細胞