RRC ID |
38406
|
著者 |
Abo M, Urano Y, Hanaoka K, Terai T, Komatsu T, Nagano T.
|
タイトル |
Development of a highly sensitive fluorescence probe for hydrogen peroxide.
|
ジャーナル |
J Am Chem Soc
|
Abstract |
Hydrogen peroxide is believed to play a role in cellular signal transduction by reversible oxidation of proteins. Here, we report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its application to imaging of hydrogen peroxide generation in live RAW 264.7 macrophages. NBzF was also employed for live cell imaging of hydrogen peroxide generated as a signaling molecule in A431 human epidermoid carcinoma cells.
|
巻・号 |
133(27)
|
ページ |
10629-37
|
公開日 |
2011-7-13
|
DOI |
10.1021/ja203521e
|
PMID |
21692459
|
MeSH |
Animals
Cell Line, Tumor
Electron Transport
Fluoresceins / analysis*
Fluoresceins / chemistry*
Fluorescence
Fluorescent Dyes / chemistry*
Humans
Hydrogen Peroxide / analysis*
Macrophages / chemistry
Mice
|
IF |
14.612
|
引用数 |
230
|
WOS 分野
|
CHEMISTRY, MULTIDISCIPLINARY
|
リソース情報 |
ヒト・動物細胞 |
|