論文 - 詳細
| RRC ID | 38415 |
|---|---|
| 著者 | Kanegae Y, Terashima M, Kondo S, Fukuda H, Maekawa A, Pei Z, Saito I. |
| タイトル | High-level expression by tissue/cancer-specific promoter with strict specificity using a single-adenoviral vector. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Tissue-/cancer-specific promoters for use in adenovirus vectors (AdVs) are valuable for elucidating specific gene functions and for use in gene therapy. However, low activity, non-specific expression and size limitations in the vector are always problems. Here, we developed a 'double-unit' AdV containing the Cre gene under the control of an α-fetoprotein promoter near the right end of its genome and bearing a compact 'excisional-expression' unit consisting of a target cDNA 'upstream' of a potent promoter between two loxPs near the left end of its genome. When Cre was expressed, the expression unit was excised as a circular molecule and strongly expressed. Undesired leak expression of Cre during virus preparation was completely suppressed by a dominant-negative Cre and a short-hairpin RNA against Cre. Using this novel construct, a very strict specificity was maintained while achieving a 40- to 90-fold higher expression level, compared with that attainable using a direct specific promoter. Therefore, the 'double-unit' AdV enabled us to produce a tissue-/cancer-specific promoter in an AdV with a high expression level and strict specificity. |
| 巻・号 | 39(2) |
| ページ | e7 |
| 公開日 | 2011-1-1 |
| DOI | 10.1093/nar/gkq966 |
| PII | gkq966 |
| PMID | 21051352 |
| PMC | PMC3025582 |
| MeSH | Adenoviridae / genetics* Cell Line Cell Line, Tumor Escherichia coli / genetics Genetic Vectors* Humans Integrases / genetics Integrases / metabolism Mutation Promoter Regions, Genetic* RNA, Small Interfering / metabolism alpha-Fetoproteins / genetics |
| IF | 11.502 |
| 引用数 | 11 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 203(RCB1637) |