| RRC ID |
38415
|
| 著者 |
Kanegae Y, Terashima M, Kondo S, Fukuda H, Maekawa A, Pei Z, Saito I.
|
| タイトル |
High-level expression by tissue/cancer-specific promoter with strict specificity using a single-adenoviral vector.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
Tissue-/cancer-specific promoters for use in adenovirus vectors (AdVs) are valuable for elucidating specific gene functions and for use in gene therapy. However, low activity, non-specific expression and size limitations in the vector are always problems. Here, we developed a 'double-unit' AdV containing the Cre gene under the control of an α-fetoprotein promoter near the right end of its genome and bearing a compact 'excisional-expression' unit consisting of a target cDNA 'upstream' of a potent promoter between two loxPs near the left end of its genome. When Cre was expressed, the expression unit was excised as a circular molecule and strongly expressed. Undesired leak expression of Cre during virus preparation was completely suppressed by a dominant-negative Cre and a short-hairpin RNA against Cre. Using this novel construct, a very strict specificity was maintained while achieving a 40- to 90-fold higher expression level, compared with that attainable using a direct specific promoter. Therefore, the 'double-unit' AdV enabled us to produce a tissue-/cancer-specific promoter in an AdV with a high expression level and strict specificity.
|
| 巻・号 |
39(2)
|
| ページ |
e7
|
| 公開日 |
2011-1-1
|
| DOI |
10.1093/nar/gkq966
|
| PII |
gkq966
|
| PMID |
21051352
|
| PMC |
PMC3025582
|
| MeSH |
Adenoviridae / genetics*
Cell Line
Cell Line, Tumor
Escherichia coli / genetics
Genetic Vectors*
Humans
Integrases / genetics
Integrases / metabolism
Mutation
Promoter Regions, Genetic*
RNA, Small Interfering / metabolism
alpha-Fetoproteins / genetics
|
| IF |
11.502
|
| 引用数 |
11
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
