| RRC ID |
38450
|
| 著者 |
Hagiyama M, Furuno T, Hosokawa Y, Iino T, Ito T, Inoue T, Nakanishi M, Murakami Y, Ito A.
|
| タイトル |
Enhanced nerve-mast cell interaction by a neuronal short isoform of cell adhesion molecule-1.
|
| ジャーナル |
J Immunol
|
| Abstract |
Close apposition of nerve and mast cells is viewed as a functional unit of neuro-immune mechanisms, and it is sustained by trans-homophilic binding of cell adhesion molecule-1 (CADM1), an Ig superfamily member. Cerebral nerve-mast cell interaction might be developmentally modulated, because the alternative splicing pattern of four (a-d) types of CADM1 transcripts drastically changed during development of the mouse cerebrum: developing cerebrums expressed CADM1b and CADM1c exclusively, while mature cerebrums expressed CADM1d additionally and predominantly. To probe how individual isoforms are involved in nerve-mast cell interaction, Neuro2a neuroblastoma cells that express CADM1c endogenously were modified to express additionally either CADM1b (Neuro2a-CADM1b) or CADM1d (Neuro2a-CADM1d), and they were cocultured with mouse bone marrow-derived mast cells (BMMCs) and BMMC-derived cell line IC-2 cells, both of which expressed CADM1c. BMMCs were found to adhere to Neuro2a-CADM1d neurites more firmly than to Neuro2a-CADM1b neurites when the adhesive strengths were estimated from the femtosecond laser-induced impulsive forces minimally required for detaching BMMCs. GFP-tagging and crosslinking experiments revealed that the firmer adhesion site consisted of an assembly of CADM1d cis-homodimers. When Neuro2a cells were specifically activated by histamine, intracellular Ca(2+) concentration was increased in 63 and 38% of CADM1c-expressing IC-2 cells that attached to the CADM1d assembly site and elsewhere, respectively. These results indicate that CADM1d is a specific neuronal isoform that enhances nerve-mast cell interaction, and they suggest that nerve-mast cell interaction may be reinforced as the brain grows mature because CADM1d becomes predominant.
|
| 巻・号 |
186(10)
|
| ページ |
5983-92
|
| 公開日 |
2011-5-15
|
| DOI |
10.4049/jimmunol.1002244
|
| PII |
jimmunol.1002244
|
| PMID |
21482734
|
| MeSH |
Alternative Splicing
Animals
Calcium / metabolism
Cell Adhesion*
Cell Adhesion Molecule-1
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / metabolism*
Cell Communication*
Cell Line, Tumor
Cells, Cultured
Cerebrum / cytology
Cerebrum / embryology
Cerebrum / growth & development
Cerebrum / immunology
Coculture Techniques
Histamine / pharmacology
Immunoglobulins / genetics
Immunoglobulins / metabolism*
Mast Cells / metabolism*
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Knockout
Neurites / metabolism
Neurons / metabolism*
Polymerase Chain Reaction
Protein Isoforms / genetics
Protein Isoforms / metabolism
|
| IF |
4.886
|
| 引用数 |
37
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
C-1300(RCB0283)
NB-1(RCB1953) |
