論文 - 詳細
| RRC ID | 38450 |
|---|---|
| 著者 | Hagiyama M, Furuno T, Hosokawa Y, Iino T, Ito T, Inoue T, Nakanishi M, Murakami Y, Ito A. |
| タイトル | Enhanced nerve-mast cell interaction by a neuronal short isoform of cell adhesion molecule-1. |
| ジャーナル | J Immunol |
| Abstract |
Close apposition of nerve and mast cells is viewed as a functional unit of neuro-immune mechanisms, and it is sustained by trans-homophilic binding of cell adhesion molecule-1 (CADM1), an Ig superfamily member. Cerebral nerve-mast cell interaction might be developmentally modulated, because the alternative splicing pattern of four (a-d) types of CADM1 transcripts drastically changed during development of the mouse cerebrum: developing cerebrums expressed CADM1b and CADM1c exclusively, while mature cerebrums expressed CADM1d additionally and predominantly. To probe how individual isoforms are involved in nerve-mast cell interaction, Neuro2a neuroblastoma cells that express CADM1c endogenously were modified to express additionally either CADM1b (Neuro2a-CADM1b) or CADM1d (Neuro2a-CADM1d), and they were cocultured with mouse bone marrow-derived mast cells (BMMCs) and BMMC-derived cell line IC-2 cells, both of which expressed CADM1c. BMMCs were found to adhere to Neuro2a-CADM1d neurites more firmly than to Neuro2a-CADM1b neurites when the adhesive strengths were estimated from the femtosecond laser-induced impulsive forces minimally required for detaching BMMCs. GFP-tagging and crosslinking experiments revealed that the firmer adhesion site consisted of an assembly of CADM1d cis-homodimers. When Neuro2a cells were specifically activated by histamine, intracellular Ca(2+) concentration was increased in 63 and 38% of CADM1c-expressing IC-2 cells that attached to the CADM1d assembly site and elsewhere, respectively. These results indicate that CADM1d is a specific neuronal isoform that enhances nerve-mast cell interaction, and they suggest that nerve-mast cell interaction may be reinforced as the brain grows mature because CADM1d becomes predominant. |
| 巻・号 | 186(10) |
| ページ | 5983-92 |
| 公開日 | 2011-5-15 |
| DOI | 10.4049/jimmunol.1002244 |
| PII | jimmunol.1002244 |
| PMID | 21482734 |
| MeSH | Alternative Splicing Animals Calcium / metabolism Cell Adhesion* Cell Adhesion Molecule-1 Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism* Cell Communication* Cell Line, Tumor Cells, Cultured Cerebrum / cytology Cerebrum / embryology Cerebrum / growth & development Cerebrum / immunology Coculture Techniques Histamine / pharmacology Immunoglobulins / genetics Immunoglobulins / metabolism* Mast Cells / metabolism* Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Knockout Neurites / metabolism Neurons / metabolism* Polymerase Chain Reaction Protein Isoforms / genetics Protein Isoforms / metabolism |
| IF | 4.886 |
| 引用数 | 37 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | C-1300(RCB0283) NB-1(RCB1953) |