| RRC ID |
38467
|
| 著者 |
Yasuda S, Hasegawa T, Hosono T, Satoh M, Watanabe K, Ono K, Shimizu S, Hayakawa T, Yamaguchi T, Suzuki K, Sato Y.
|
| タイトル |
AW551984: a novel regulator of cardiomyogenesis in pluripotent embryonic cells.
|
| ジャーナル |
Biochem J
|
| Abstract |
An understanding of the mechanism that regulates the cardiac differentiation of pluripotent stem cells is necessary for the effective generation and expansion of cardiomyocytes as cell therapy products. In the present study, we have identified genes that modulate the cardiac differentiation of pluripotent embryonic cells. We isolated P19CL6 cell sublines that possess distinct properties in cardiomyogenesis and extracted 24 CMR (cardiomyogenesis-related candidate) genes correlated with cardiomyogenesis using a transcriptome analysis. Knockdown of the CMR genes by RNAi (RNA interference) revealed that 18 genes influence spontaneous contraction or transcript levels of cardiac marker genes in EC (embryonal carcinoma) cells. We also performed knockdown of the CMR genes in mouse ES (embryonic stem) cells and induced in vitro cardiac differentiation. Three CMR genes, AW551984, 2810405K02Rik (RIKEN cDNA 2810405K02 gene) and Cd302 (CD302 antigen), modulated the cardiac differentiation of both EC cells and ES cells. Depletion of AW551984 attenuated the expression of the early cardiac transcription factor Nkx2.5 (NK2 transcription factor related locus 5) without affecting transcript levels of pluripotency and early mesoderm marker genes during ES cell differentiation. Activation of Wnt/β-catenin signalling enhanced the expression of both AW551984 and Nkx2.5 in ES cells during embryoid body formation. Our findings indicate that AW551984 is a novel regulator of cardiomyogenesis from pluripotent embryonic cells, which links Wnt/β-catenin signalling to Nkx2.5 expression.
|
| 巻・号 |
437(2)
|
| ページ |
345-55
|
| 公開日 |
2011-7-15
|
| DOI |
10.1042/BJ20110520
|
| PII |
BJ20110520
|
| PMID |
21554246
|
| MeSH |
Animals
Cell Differentiation / drug effects*
Embryonic Stem Cells / metabolism
Gene Expression Regulation, Developmental
Heart / embryology*
Homeobox Protein Nkx-2.5
Homeodomain Proteins / biosynthesis
Mice
Myocytes, Cardiac / metabolism
Myocytes, Cardiac / physiology*
Pluripotent Stem Cells / drug effects
Pluripotent Stem Cells / metabolism*
RNA Interference
Transcription Factors / biosynthesis
Transcription Factors / genetics
Transcription Factors / physiology*
Wnt Proteins / physiology
|
| IF |
4.097
|
| 引用数 |
4
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
P19CL6 |
