論文 - 詳細
| RRC ID | 38471 |
|---|---|
| 著者 | Otani T, Tsuji-Takayama K, Okochi A, Yamamoto M, Takeuchi M, Yamasaki F, Nakamura S, Kibata M. |
| タイトル | Stromal cells' B7-1 is a key stimulatory molecule for interleukin-10 production by HOZOT, a multifunctional regulatory T-cell line. |
| ジャーナル | Immunol Cell Biol |
| Abstract |
We have previously shown that xenogeneic stromal cell stimulation of naïve T cells resulted in the generation of a new type of regulatory T (Treg) cell termed HOZOT, which has multifunctional properties and a CD4/CD8 double-positive phenotype. Even after the establishment of HOZOT, stromal cells can function as an antigen-presenting cell (APC) by inducing these cells to produce interleukin (IL)-10. When compared with other stimuli, stromal cells showed an IL-10-producing ability comparable to anti-CD3 antibody (Ab) stimulation, and much greater than dendritic cell (DC) stimulation. Distinct from professional APCs, stromal cells express only major histocompatibility complex (MHC) class I and B7-1 costimulatory molecules, and not MHC class II or other costimulatory molecules, such as ICOSL (CD275), PD-L1 (CD274), PD-L2 (CD273), CD40, OX40L (CD252) and 4-1BBL (CD137L) in the absence of stimulation. Blocking experiments revealed that, in addition to anti-H-2K(d) Ab and anti-human CD8 Ab, anti-mouse B7-1 Ab could effectively block IL-10 production, indicating a key role of the B7-1/CD28 pathway. Using stromal cells expressing different levels of B7-1, IL-10 production correlated with the levels of B7-1 expression. Distinct from ICOSL or PD-L1 expressed on DCs (which are regarded as IL-10-inducing costimulatory molecules), this study showed that B7-1 on stromal cells is a key molecule regulating IL-10 production by multifunctional Treg cells, HOZOT. |
| 巻・号 | 89(2) |
| ページ | 246-54 |
| 公開日 | 2011-2-1 |
| DOI | 10.1038/icb.2010.87 |
| PII | icb201087 |
| PMID | 20628373 |
| MeSH | Animals Antibodies, Monoclonal / biosynthesis B7-1 Antigen / immunology* CD8 Antigens / metabolism Cell Line Dendritic Cells / cytology Dendritic Cells / immunology Histocompatibility Antigens Class I / immunology Humans Interleukin-10 / biosynthesis* Mice Phenotype Stromal Cells / cytology Stromal Cells / immunology T-Lymphocytes, Regulatory / cytology* T-Lymphocytes, Regulatory / immunology* |
| IF | 3.764 |
| 引用数 | 2 |
| WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-G2/PA6(RCB1127) |