RRC ID 38502
著者 Miyata M, Kambe M, Tajima O, Moriya S, Sawaki H, Hotta H, Kondo Y, Narimatsu H, Miyagi T, Furukawa K, Furukawa K.
タイトル Membrane sialidase NEU3 is highly expressed in human melanoma cells promoting cell growth with minimal changes in the composition of gangliosides.
ジャーナル Cancer Sci
Abstract NEU3 is a membrane sialidase specific for gangliosides. Its increased expression and implication in some cancers have been reported. Here, we analyzed NEU3 expression in malignant melanoma cell lines and its roles in the cancer phenotypes. Quantitative RT-PCR revealed that high levels of the NEU3 gene were expressed at almost equivalent levels with those in colon cancers. To examine the effects of overexpression of NEU3, NEU3 cDNA-transfectant cells were established using a melanoma cell line SK-MEL-28 and its mutant N1 lacking GD3. SK-MEL-28 sublines overexpressing both the NEU3 gene and NEU3 enzyme activity showed no changes in both cell growth and ganglioside expression, while N1 cells showed a mild increase in cell proliferation with increased phosphorylation of the EGF receptor and neo-synthesis of Gb3 after NEU3 transfection. In contrast, NEU3 silencing resulted in a definite reduction in cell growth in a melanoma line MeWo, while ganglioside patterns underwent minimal changes. Phosphorylation levels of ERK1/2 with serum stimulation decreased in the NEU3-silenced cells. All these results suggest that NEU3 is highly expressed to enhance malignant phenotypes including apoptosis inhibition in malignant melanomas.
巻・号 102(12)
ページ 2139-49
公開日 2011-12-1
DOI 10.1111/j.1349-7006.2011.02086.x
PMID 21895867
MeSH Antigens, Tumor-Associated, Carbohydrate / biosynthesis Apoptosis Cell Line, Tumor Cell Proliferation* Colonic Neoplasms / metabolism ErbB Receptors / metabolism Gangliosides / metabolism* Humans Melanoma / genetics Melanoma / metabolism* Melanoma / pathology* Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Neoplasm Proteins / biosynthesis Neoplasm Proteins / genetics Neuraminidase / biosynthesis* Neuraminidase / genetics RNA Interference RNA, Small Interfering
IF 4.966
引用数 23
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 COLO-320(RCB1193) CACO-2(RCB0988) LoVo(RCB1639)