論文 - 詳細
| RRC ID | 38524 |
|---|---|
| 著者 | Ando Y, Tomaru Y, Morinaga A, Burroughs AM, Kawaji H, Kubosaki A, Kimura R, Tagata M, Ino Y, Hirano H, Chiba J, Suzuki H, Carninci P, Hayashizaki Y. |
| タイトル | Nuclear pore complex protein mediated nuclear localization of dicer protein in human cells. |
| ジャーナル | PLoS One |
| Abstract |
Human DICER1 protein cleaves double-stranded RNA into small sizes, a crucial step in production of single-stranded RNAs which are mediating factors of cytoplasmic RNA interference. Here, we clearly demonstrate that human DICER1 protein localizes not only to the cytoplasm but also to the nucleoplasm. We also find that human DICER1 protein associates with the NUP153 protein, one component of the nuclear pore complex. This association is detected predominantly in the cytoplasm but is also clearly distinguishable at the nuclear periphery. Additional characterization of the NUP153-DICER1 association suggests NUP153 plays a crucial role in the nuclear localization of the DICER1 protein. |
| 巻・号 | 6(8) |
| ページ | e23385 |
| 公開日 | 2011-1-1 |
| DOI | 10.1371/journal.pone.0023385 |
| PII | PONE-D-11-06216 |
| PMID | 21858095 |
| PMC | PMC3156128 |
| MeSH | Active Transport, Cell Nucleus Amino Acid Sequence Blotting, Western Cell Line Cell Nucleus / metabolism* DEAD-box RNA Helicases / genetics DEAD-box RNA Helicases / metabolism* HEK293 Cells HeLa Cells Humans Immunoprecipitation Microscopy, Confocal Nuclear Pore Complex Proteins / genetics Nuclear Pore Complex Proteins / metabolism* Protein Binding RNA Interference Ribonuclease III / genetics Ribonuclease III / metabolism* |
| IF | 2.74 |
| 引用数 | 30 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | NB1RGB(RCB0222) |