| RRC ID |
38528
|
| Author |
Akagi T, Shimizu K, Takahama S, Iwasaki T, Sakamaki K, Endo Y, Sawasaki T.
|
| Title |
Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling.
|
| Journal |
FEBS Lett
|
| Abstract |
We screened a library of human single-transmembrane proteins (sTMPs), produced by a cell-free system, using a luminescent assay to identify those that can be cleaved by caspase-8 (CASP8). Of the 407 sTMPs screened, only the interleukin-21 receptor (IL21R), vezatin (VEZT), and carbonic anhydrase XIV were cleaved at Asp344, Asp655 and Asp53, respectively. We confirmed that IL21R and VEZT were also cleaved in apoptotic HeLa cells with the cleavage sites. Interestingly, IL21R was cleaved within 30 min after apoptosis induction. Furthermore the CASP8-cleaved form of IL21R did not induce phosphorylation at Tyr705 of STAT3. Our results suggest that the interleukin-21 signaling cascade is negatively regulated by CASP8.
|
| Volume |
585(12)
|
| Pages |
1835-40
|
| Published |
2011-6-23
|
| DOI |
10.1016/j.febslet.2011.04.031
|
| PII |
S0014-5793(11)00278-X
|
| PMID |
21524651
|
| MeSH |
Apoptosis*
Carrier Proteins / metabolism
Caspase 8 / metabolism*
Feedback, Physiological*
HeLa Cells
Humans
Interleukins / metabolism*
Membrane Proteins / metabolism
Peptide Library
Receptors, Interleukin-21 / metabolism*
STAT3 Transcription Factor
Signal Transduction*
|
| IF |
3.057
|
| Times Cited |
6
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
