論文 - 詳細
| RRC ID | 38553 |
|---|---|
| 著者 | Zhang W, Tsurushima H, Oyane A, Yazaki Y, Sogo Y, Ito A, Matsumura A. |
| タイトル | BMP-2 gene-fibronectin-apatite composite layer enhances bone formation. |
| ジャーナル | J Biomed Sci |
| Abstract |
BACKGROUND:Safe and efficient gene transfer systems are needed for tissue engineering. We have developed an apatite composite layer including the bone morphogenetic protein-2 (BMP-2) gene and fibronectin (FB), and we evaluated its ability to induce bone formation. METHODS:An apatite composite layer was evaluated to determine the efficiency of gene transfer to cells cultured on it. Cells were cultured on a composite layer including the BMP-2 gene and FB, and BMP-2 gene expression, BMP-2 protein concentrations, alkaline phosphatase (ALP) activity, and osteocalcin (OC) concentrations were measured. A bone defect on the cranium of rats was treated with hydroxyapatite (HAP)-coated ceramic buttons with the apatite composite layer including the BMP-2 gene and FB (HAP-BMP-FB). The tissue concentration of BMP-2, bone formation, and the expression levels of the BMP-2, ALP, and OC genes were all quantified. RESULTS:The apatite composite layer provided more efficient gene transfer for the cultured cells than an apatite composite layer without FB. The BMP-2 concentration was approximately 100-600 pg/mL in the cell-culture medium. Culturing the cells on the apatite composite layer for 27 days increased ALP activity and OC concentrations. In animal experiments, the tissue concentrations of BMP-2 were over 100 pg/mg in the HAP-BMP-FB group and approximately 50 pg/mg in the control groups. Eight weeks later, bone formation was more enhanced in the HAP-BMP-FB group than in the control groups. In the tissues surrounding the HAP button, the gene expression levels of ALP and OC increased. CONCLUSION:The BMP-2 gene-FB-apatite composite layer might be useful for bone engineering. |
| 巻・号 | 18(1) |
| ページ | 62 |
| 公開日 | 2011-8-23 |
| DOI | 10.1186/1423-0127-18-62 |
| PII | 1423-0127-18-62 |
| PMID | 21859498 |
| PMC | PMC3175450 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism Bone Morphogenetic Protein 2 / physiology* Calcium Phosphates / metabolism Cells, Cultured DNA Primers / genetics Durapatite / metabolism* Enzyme-Linked Immunosorbent Assay Fibronectins / physiology* Gene Transfer Techniques* HeLa Cells Humans Membrane Proteins / metabolism Mice Microscopy, Electron, Scanning Osteocalcin / metabolism Osteogenesis / genetics Osteogenesis / physiology* Plasmids / genetics Rats Reverse Transcriptase Polymerase Chain Reaction Skull / injuries Skull / surgery Tissue Engineering / methods* |
| IF | 5.762 |
| 引用数 | 24 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) 10T1/2(RCB0247) HeLa(RCB0007) |