論文 - 詳細
| RRC ID | 38559 |
|---|---|
| 著者 | Kobayashi T, Machida K, Mikami S, Masutani M, Imataka H. |
| タイトル | Cell-free RNA replication systems based on a human cell extracts-derived in vitro translation system with the encephalomyocarditisvirus RNA. |
| ジャーナル | J Biochem |
| Abstract |
To study the relationship between translation and replication of encephalomyocarditisvirus (EMCV) RNA, we established a cell-free RNA replication system by employing a human cell extracts-based in vitro translation system. In this system, a cis-EMCV RNA replicon encoding the Renilla luciferase (R-luc) or GFP and the viral regulatory proteins efficiently replicated with simultaneous translation of the encoded protein. To examine how translation of the replicon RNA, but not the translated products, affected replication, a trans-EMCV RNA replicon encoding R-luc and the RNA replication elements was next constructed. The trans-replicon RNA replicated only in the presence of the regulatory proteins pre-expressed in trans. Incubation with cycloheximide, puromycin or a dominant-negative eukaryotic translation initiation factor 4A following expression of the regulatory proteins almost completely inhibited not only translation of the trans-replicon RNA but also replication of the RNA, suggesting that EMCV RNA translation promotes replication of the RNA. In conclusion, the cell-free RNA replication systems should become useful tools for the study of the viral RNA replication. |
| 巻・号 | 150(4) |
| ページ | 423-30 |
| 公開日 | 2011-10-1 |
| DOI | 10.1093/jb/mvr072 |
| PII | mvr072 |
| PMID | 21622667 |
| MeSH | Animals Cell Extracts / pharmacology* Cell-Free System Cells, Cultured Cricetinae Cycloheximide / pharmacology Encephalomyocarditis virus / drug effects* Encephalomyocarditis virus / genetics* Encephalomyocarditis virus / metabolism HeLa Cells Humans Puromycin / pharmacology RNA, Viral / biosynthesis* RNA, Viral / genetics Virus Replication / drug effects* Virus Replication / genetics |
| IF | 2.476 |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa S3 |