| RRC ID |
38578
|
| Author |
Nagahama Y, Obama T, Usui M, Kanazawa Y, Iwamoto S, Suzuki K, Miyazaki A, Yamaguchi T, Yamamoto M, Itabe H.
|
| Title |
Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E(2) (PGE(2)) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE(2)-producing enzymes, cyclooxygenase-2 and microsomal PGE(2) synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-κB) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-κB pathway. These data suggest a mechanistic link between periodontal disease and lipid metabolism-related disorders, including atherosclerosis.
|
| Volume |
413(4)
|
| Pages |
566-71
|
| Published |
2011-10-7
|
| DOI |
10.1016/j.bbrc.2011.09.002
|
| PII |
S0006-291X(11)01573-7
|
| PMID |
21925143
|
| MeSH |
CD36 Antigens / biosynthesis
Cell Line
Chronic Periodontitis / chemically induced
Chronic Periodontitis / enzymology*
Cyclooxygenase 2 / metabolism*
Dinoprostone / biosynthesis
Gingiva / drug effects
Gingiva / enzymology*
Humans
Interleukin-8 / biosynthesis
Intramolecular Oxidoreductases / metabolism*
Keratinocytes / drug effects
Keratinocytes / enzymology
Lipoproteins, LDL / metabolism*
Lipoproteins, LDL / pharmacology
Microsomes / enzymology
Mouth Mucosa / drug effects
Mouth Mucosa / enzymology
NF-kappa B / metabolism
Prostaglandin-E Synthases
Up-Regulation
|
| IF |
2.985
|
| Times Cited |
11
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
