RRC ID 38578
著者 Nagahama Y, Obama T, Usui M, Kanazawa Y, Iwamoto S, Suzuki K, Miyazaki A, Yamaguchi T, Yamamoto M, Itabe H.
タイトル Oxidized low-density lipoprotein-induced periodontal inflammation is associated with the up-regulation of cyclooxygenase-2 and microsomal prostaglandin synthase 1 in human gingival epithelial cells.
ジャーナル Biochem Biophys Res Commun
Abstract Periodontitis is characterized by chronic gingival tissue inflammation, and inflammatory mediators such as IL-8 and prostaglandin E(2) (PGE(2)) are associated with disease progression. Previously we showed that oxidatively modified low-density lipoprotein (oxLDL) was present in gingival crevicular fluid. In this study, the role of oxLDL in the gingival epithelial cell inflammatory response was further investigated using Ca9-22 cells and primary human oral keratinocytes (HOK). Treatment of Ca9-22 cells and HOK with oxLDL induced an up-regulation of IL-8 and the PGE(2)-producing enzymes, cyclooxygenase-2 and microsomal PGE(2) synthase-1. These responses induced by oxLDL were significantly suppressed by a nuclear factor-kappa B (NF-κB) inhibitor. However, unlike the result in macrophages, oxLDL did not lead to an increase in CD36 expression in these two cells. These results suggest that oxLDL elicits gingival epithelial cell inflammatory responses through an activation of the NF-κB pathway. These data suggest a mechanistic link between periodontal disease and lipid metabolism-related disorders, including atherosclerosis.
巻・号 413(4)
ページ 566-71
公開日 2011-10-7
DOI 10.1016/j.bbrc.2011.09.002
PII S0006-291X(11)01573-7
PMID 21925143
MeSH CD36 Antigens / biosynthesis Cell Line Chronic Periodontitis / chemically induced Chronic Periodontitis / enzymology* Cyclooxygenase 2 / metabolism* Dinoprostone / biosynthesis Gingiva / drug effects Gingiva / enzymology* Humans Interleukin-8 / biosynthesis Intramolecular Oxidoreductases / metabolism* Keratinocytes / drug effects Keratinocytes / enzymology Lipoproteins, LDL / metabolism* Lipoproteins, LDL / pharmacology Microsomes / enzymology Mouth Mucosa / drug effects Mouth Mucosa / enzymology NF-kappa B / metabolism Prostaglandin-E Synthases Up-Regulation
IF 2.985
引用数 11
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞