| RRC ID |
38585
|
| 著者 |
Tsuruno C, Okuma K, Takahashi Y, Tanaka R, Tanaka Y, Takahama Y, Hamaguchi Y, Hamaguchi I, Yamaguchi K.
|
| タイトル |
A recombinant vesicular stomatitis virus encoding HIV-1 receptors and human OX40 ligand efficiently eliminates HIV-1-infected CD4-positive T cells expressing OX40.
|
| ジャーナル |
Hum Immunol
|
| Abstract |
OX40 protein is highly expressed on activated CD4-positive T cells that are susceptible for human immunodeficiency virus type 1 (HIV-1) infection. To target and kill HIV-1-infected OX40(+) T cells, we used a recombinant vesicular stomatitis virus (rVSV) lacking its envelope glycoprotein (ΔG) and instead expressing HIV-1 receptors CD4/CXCR4 and OX40 ligand (OX40L). Expression of OX40L as well as HIV-1 receptors on the VSV particles led to specific infection of OX40(+) T cells, including primary cells, either acutely or chronically infected with X4 HIV-1. Consequently, the rVSV rapidly eliminated these infected cells and caused a marked reduction of HIV-1 viral load in culture. Inclusion of the OX40L gene in the VSV recombinant led to significantly better infection and HIV-1 elimination compared with an rVSVΔG expressing only HIV-1 receptors. A novel rVSVΔG encoding both HIV-1 receptors and OX40L has a potentially greater therapeutic value than an rVSVΔG expressing only HIV-1 receptors.
|
| 巻・号 |
72(4)
|
| ページ |
295-304
|
| 公開日 |
2011-4-1
|
| DOI |
10.1016/j.humimm.2011.01.007
|
| PII |
S0198-8859(11)00015-2
|
| PMID |
21262309
|
| MeSH |
Animals
CD4 Antigens / genetics
CD4 Antigens / immunology
CD4 Antigens / metabolism
CD4-Positive T-Lymphocytes / immunology*
CD4-Positive T-Lymphocytes / virology*
Cell Line
Cell Line, Tumor
Cricetinae
Gene Order
Genetic Vectors / genetics
Genetic Vectors / isolation & purification
Genetic Vectors / metabolism
Giant Cells / metabolism
Giant Cells / pathology
HEK293 Cells
HIV Infections* / immunology
HIV Infections* / virology
HIV-1 / immunology*
HIV-1 / metabolism
Humans
OX40 Ligand / genetics
OX40 Ligand / immunology*
Receptors, CXCR4 / genetics
Receptors, CXCR4 / immunology
Receptors, CXCR4 / metabolism
Receptors, OX40 / immunology*
Vesiculovirus / genetics
Vesiculovirus / isolation & purification
Vesiculovirus / metabolism*
Viral Load
|
| IF |
2.412
|
| 引用数 |
4
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
BHK-21(RCB1423) |
