| RRC ID |
3859
|
| 著者 |
Ito T, Maki N, Hazeki O, Sasaki K, Nekooki M.
|
| タイトル |
Extracellular and transmembrane region of a podocalyxin-like protein 1 fragment identified from colon cancer cell lines.
|
| ジャーナル |
Cell Biol Int
|
| Abstract |
Regulated intramembrane proteolysis of membrane proteins has been shown to play an important role in cell differentiation and in the pathogenesis of diseases. The aim of the present study was to identify novel peptides generated by intramembrane proteolysis. The peptides were identified in serum-free cultured (SFC) media from various cell lines by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). A 2315-Da peptide found only in medium from SFC colon cancer cell lines was identified and shown to consist of a portion of both the extracellular and transmembrane regions of human podocalyxin-like 1. This protein fragment was not found in lung or pancreatic cancer cell lines by immunoprecipitation-SELDI tests using an antibody specific to this fragment, suggesting that this human podocalyxin-like protein 1 fragment may be unique to colon cancer cell lines.
|
| 巻・号 |
31(12)
|
| ページ |
1518-24
|
| 公開日 |
2007-12-1
|
| DOI |
10.1016/j.cellbi.2007.06.017
|
| PII |
S1065-6995(07)00190-4
|
| PMID |
17719804
|
| MeSH |
Amino Acid Sequence
Animals
Biomarkers, Tumor / isolation & purification*
Cell Line, Tumor
Colonic Neoplasms / chemistry*
Colonic Neoplasms / metabolism
Culture Media, Serum-Free / analysis
Humans
Membrane Proteins / isolation & purification*
Membrane Proteins / metabolism
Mice
Mice, Inbred BALB C
Neoplasm Proteins / isolation & purification*
Peptide Mapping*
Protein Structure, Tertiary
Sialoglycoproteins / isolation & purification*
Sialoglycoproteins / metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
|
| IF |
2.571
|
| 引用数 |
5
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CACO-2(RCB0988)
LC-2/ad(RCB0440) |
