論文 - 詳細
| RRC ID | 3859 |
|---|---|
| 著者 | Ito T, Maki N, Hazeki O, Sasaki K, Nekooki M. |
| タイトル | Extracellular and transmembrane region of a podocalyxin-like protein 1 fragment identified from colon cancer cell lines. |
| ジャーナル | Cell Biol Int |
| Abstract |
Regulated intramembrane proteolysis of membrane proteins has been shown to play an important role in cell differentiation and in the pathogenesis of diseases. The aim of the present study was to identify novel peptides generated by intramembrane proteolysis. The peptides were identified in serum-free cultured (SFC) media from various cell lines by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). A 2315-Da peptide found only in medium from SFC colon cancer cell lines was identified and shown to consist of a portion of both the extracellular and transmembrane regions of human podocalyxin-like 1. This protein fragment was not found in lung or pancreatic cancer cell lines by immunoprecipitation-SELDI tests using an antibody specific to this fragment, suggesting that this human podocalyxin-like protein 1 fragment may be unique to colon cancer cell lines. |
| 巻・号 | 31(12) |
| ページ | 1518-24 |
| 公開日 | 2007-12-1 |
| DOI | 10.1016/j.cellbi.2007.06.017 |
| PII | S1065-6995(07)00190-4 |
| PMID | 17719804 |
| MeSH | Amino Acid Sequence Animals Biomarkers, Tumor / isolation & purification* Cell Line, Tumor Colonic Neoplasms / chemistry* Colonic Neoplasms / metabolism Culture Media, Serum-Free / analysis Humans Membrane Proteins / isolation & purification* Membrane Proteins / metabolism Mice Mice, Inbred BALB C Neoplasm Proteins / isolation & purification* Peptide Mapping* Protein Structure, Tertiary Sialoglycoproteins / isolation & purification* Sialoglycoproteins / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods |
| IF | 2.571 |
| 引用数 | 5 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) LC-2/ad(RCB0440) |