論文 - 詳細
| RRC ID | 38590 |
|---|---|
| 著者 | Kaida A, Sawai N, Sakaguchi K, Miura M. |
| タイトル | Fluorescence kinetics in HeLa cells after treatment with cell cycle arrest inducers visualized with Fucci (fluorescent ubiquitination-based cell cycle indicator). |
| ジャーナル | Cell Biol Int |
| Abstract |
Fucci (fluorescent ubiquitination-based cell cycle indicator) is able to visualize dynamics of cell cycle progression in live cells; G1- and S-/G2-/M-phase cells expressing Fucci emit red and green fluorescence, respectively. This system could be applied to cell kinetic analysis of tumour cells in the field of cancer therapy; however, it is still unclear how fluorescence kinetics change after various treatments, including exposure to anticancer agents. To explore this, we arrested live HeLa cells expressing the Fucci probes at various cell cycle stages and observed the fluorescence, in conjunction with flow cytometric analysis. X-irradiation, HU (hydroxyurea) and nocodazole arrest cells at G2/M boundary, early S-phase and early M-phase, respectively. Although X-irradiation and HU treatment induced similar accumulation kinetics of green fluorescent cells, nocodazole treatment induced an abnormal red fluorescence at M phase, followed by accumulation of both red and green fluorescent cells with 4N DNA content. We conclude that certain agents that disrupt normal cell cycle regulation could cause unexpected fluorescence kinetics in the Fucci system. |
| 巻・号 | 35(4) |
| ページ | 359-63 |
| 公開日 | 2011-4-1 |
| DOI | 10.1042/CBI20100643 |
| PII | CBI20100643 |
| PMID | 21231917 |
| MeSH | Cell Cycle / drug effects* Cell Division / drug effects Cell Survival Flow Cytometry Fluorescence Fluorescent Dyes / analysis* G1 Phase / drug effects G2 Phase / drug effects Gene Expression HeLa Cells / cytology* HeLa Cells / drug effects HeLa Cells / metabolism Humans Indicators and Reagents Microscopy, Fluorescence Proteins / analysis* Proteins / genetics Ubiquitination |
| IF | 2.571 |
| 引用数 | 21 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |