論文 - 詳細
| RRC ID | 38592 |
|---|---|
| 著者 | Nishigaki T, Teramura Y, Nasu A, Takada K, Toguchida J, Iwata H. |
| タイトル | Highly efficient cryopreservation of human induced pluripotent stem cells using a dimethyl sulfoxide-free solution. |
| ジャーナル | Int J Dev Biol |
| Abstract |
Human induced pluripotent stem (hiPS) cells have great potential for regenerative medicine and drug discovery. It is essential to establish highly efficient and reliable methods for hiPS cell cryopreservation. We examined cryopreservation of hiPS cells by the vitrification method using a dimethyl sulfoxide Me2SO-free and serum-free medium, VS2E, that uses Euro-Collins solution as a base with 40% (v/v) ethylene glycol and 10% (w/v) polyethylene glycol as cryoprotectants. This combination of vitrification and cryoprotectants resulted in a higher recovery rate of hiPS cells than with a commercially-available vitrification solution, DAP213, which contained Me2SO and serum components. After vitrification and warming, hiPS cells were cultured easily. Even after several subculturing steps, cells expressed undifferentiated cell markers, such as Oct-3/4 and SSEA-4, and also exhibited alkaline phosphatase activity. The pluripotency of hiPS cells was maintained, as demonstrated by teratoma formation upon hiPS cell transplantation into severe combined immunodeficient mice. Thus, we successfully preserved hiPS cells under liquid nitrogen with high efficiency using Me2SO-free vitrification solution and rapid cooling. |
| 巻・号 | 55(3) |
| ページ | 305-11 |
| 公開日 | 2011-1-1 |
| DOI | 10.1387/ijdb.103145tn |
| PII | 103145tn |
| PMID | 21710436 |
| MeSH | Acetamides Alkaline Phosphatase / biosynthesis Alkaline Phosphatase / metabolism Animals Cell Survival / drug effects Cryopreservation / methods* Cryoprotective Agents / pharmacology* Dimethyl Sulfoxide / pharmacology Ethylene Glycol / pharmacology Humans Hypertonic Solutions / pharmacology Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / drug effects Induced Pluripotent Stem Cells / metabolism* Mice Mice, SCID Octamer Transcription Factor-3 / biosynthesis Polyethylene Glycols / pharmacology Propylene Glycols Stage-Specific Embryonic Antigens / biosynthesis |
| IF | 1.105 |
| 引用数 | 14 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |