論文 - 詳細
| RRC ID | 38597 |
|---|---|
| 著者 | Shinozaki K, Hosokawa Y, Hazawa M, Kashiwakura I, Okumura K, Kaku T, Nakayama E. |
| タイトル | Ascorbic acid enhances radiation-induced apoptosis in an HL60 human leukemia cell line. |
| ジャーナル | J Radiat Res |
| Abstract |
This study was conducted to examine the utility of the combined use of ascorbic acid (AsA) and radiation in clinical applications. We investigated cell survival, DNA fragmentation, and caspase activation after X-ray irradiation and AsA treatment of human leukemia HL60 cells. The number of living cells decreased after combined X-ray irradiation and AsA treatment (2 Gy + 5 mM) in comparison with that after X-ray irradiation (2 Gy) or AsA treatment (5 mM) alone. DNA fragmentation was more in the cells subjected to combined X-ray irradiation and AsA treatment than in those subjected to X-ray irradiation alone. Caspase-3, caspase-8, and caspase-9 were highly activated following combined X-ray irradiation and AsA treatment, but caspase-8 activity was not markedly increased after X-ray irradiation alone. Bax levels in the mitochondrial membrane fractions were increased after AsA treatment alone and after combined X-ray irradiation and AsA treatment. However, there was no apparent increase in the Bax levels after X-ray irradiation treatment alone. Thus, this study confirmed that supplementing X-ray irradiation with AsA treatment results in increased apoptosis in HL60 cells. With regard to the apoptosis-inducing factors, we hypothesized that Bax and caspase-8 were activated after combined X-ray irradiation and AsA treatment compared with either treatment alone. |
| 巻・号 | 52(2) |
| ページ | 229-37 |
| 公開日 | 2011-1-1 |
| DOI | 10.1269/jrr.10089 |
| PII | JST.JSTAGE/jrr/10089 |
| PMID | 21343676 |
| MeSH | Apoptosis* Ascorbic Acid / pharmacology* Caspase 3 / metabolism Caspase 8 / metabolism Caspase 9 / metabolism Cell Line, Tumor Cell Survival DNA Fragmentation HL-60 Cells Humans Leukemia / drug therapy* Leukemia / pathology Leukemia / radiotherapy* Mitochondria / metabolism Time Factors X-Rays bcl-2-Associated X Protein / metabolism |
| IF | 1.95 |
| 引用数 | 29 |
| WOS 分野 | BIOLOGY RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) |