論文 - 詳細
| RRC ID | 38600 |
|---|---|
| 著者 | Ito M, Kitamura H, Kikuguchi C, Hase K, Ohno H, Ohara O. |
| タイトル | SP600125 inhibits cap-dependent translation independently of the c-Jun N-terminal kinase pathway. |
| ジャーナル | Cell Struct Funct |
| Abstract |
We investigated the effects of SP600125 (formerly called c-Jun N-terminal kinase (JNK) inhibitor II) on translation using cultured mouse cells. SP600125 (50 µM) treatment rapidly repressed overall protein synthesis, accompanied by a reduction in the mRNAs for housekeeping genes such as glyceraldehyde-3-phosphate dehydrogenase in the polysomal fraction. SP600125 decreased polysomes with a concomitant increase in free ribosomal subunits in the cytoplasm, suggesting that global translation was inhibited at the initiation step. A reporter analysis using exogenous mRNAs showed that SP600125 inhibited cap-dependent but not internal ribosome entry site-dependent translation. SP600125 significantly attenuated phosphorylation of components in the mTOR pathway, which is responsible for cap-dependent translation. In contrast to SP600125, short hairpin RNAs for JNK1 and JNK2 failed to affect overall protein synthesis. Collectively, SP600125 inhibits cap-dependent translation, independent of the JNK pathway. |
| 巻・号 | 36(1) |
| ページ | 27-33 |
| 公開日 | 2011-1-1 |
| DOI | 10.1247/csf.10025 |
| PII | JST.JSTAGE/csf/10025 |
| PMID | 21263197 |
| MeSH | 3T3 Cells Animals Anthracenes / pharmacology* Gene Knockdown Techniques JNK Mitogen-Activated Protein Kinases / deficiency JNK Mitogen-Activated Protein Kinases / genetics JNK Mitogen-Activated Protein Kinases / metabolism* Mice Protein Biosynthesis / drug effects* Protein Kinase Inhibitors / pharmacology* RNA, Messenger / genetics Signal Transduction / drug effects |
| IF | 2.429 |
| 引用数 | 2 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 3T3 Swiss Albino(RCB1642) |