| RRC ID |
38600
|
| 著者 |
Ito M, Kitamura H, Kikuguchi C, Hase K, Ohno H, Ohara O.
|
| タイトル |
SP600125 inhibits cap-dependent translation independently of the c-Jun N-terminal kinase pathway.
|
| ジャーナル |
Cell Struct Funct
|
| Abstract |
We investigated the effects of SP600125 (formerly called c-Jun N-terminal kinase (JNK) inhibitor II) on translation using cultured mouse cells. SP600125 (50 µM) treatment rapidly repressed overall protein synthesis, accompanied by a reduction in the mRNAs for housekeeping genes such as glyceraldehyde-3-phosphate dehydrogenase in the polysomal fraction. SP600125 decreased polysomes with a concomitant increase in free ribosomal subunits in the cytoplasm, suggesting that global translation was inhibited at the initiation step. A reporter analysis using exogenous mRNAs showed that SP600125 inhibited cap-dependent but not internal ribosome entry site-dependent translation. SP600125 significantly attenuated phosphorylation of components in the mTOR pathway, which is responsible for cap-dependent translation. In contrast to SP600125, short hairpin RNAs for JNK1 and JNK2 failed to affect overall protein synthesis. Collectively, SP600125 inhibits cap-dependent translation, independent of the JNK pathway.
|
| 巻・号 |
36(1)
|
| ページ |
27-33
|
| 公開日 |
2011-1-1
|
| DOI |
10.1247/csf.10025
|
| PII |
JST.JSTAGE/csf/10025
|
| PMID |
21263197
|
| MeSH |
3T3 Cells
Animals
Anthracenes / pharmacology*
Gene Knockdown Techniques
JNK Mitogen-Activated Protein Kinases / deficiency
JNK Mitogen-Activated Protein Kinases / genetics
JNK Mitogen-Activated Protein Kinases / metabolism*
Mice
Protein Biosynthesis / drug effects*
Protein Kinase Inhibitors / pharmacology*
RNA, Messenger / genetics
Signal Transduction / drug effects
|
| IF |
2.429
|
| 引用数 |
2
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
3T3 Swiss Albino(RCB1642) |
