RRC ID 38612
著者 Horie M, Fukui H, Nishio K, Endoh S, Kato H, Fujita K, Miyauchi A, Nakamura A, Shichiri M, Ishida N, Kinugasa S, Morimoto Y, Niki E, Yoshida Y, Iwahashi H.
タイトル Evaluation of acute oxidative stress induced by NiO nanoparticles in vivo and in vitro.
ジャーナル J Occup Health
Abstract OBJECTIVES:Nickel oxide (NiO) is an important industrial material, and it is also a harmful agent. The toxicity of NiO is size-related: nanoparticles are more toxic than fine-particles. The toxic mechanism induced by NiO nanoparticles remains unexplained, and the relationship between in vitro and in vivo NiO toxicity results is unclear. In the present study, we focused on the oxidative stress caused by NiO nanoparticles by examining and comparing in vitro and in vivo acute responses induced by NiO nanoparticles.
METHODS:Cellular responses induced by black NiO nanoparticles with a primary particle size of 20 nm, were examined in human lung carcinoma A549 cells. In vivo responses were examined by instillation of NiO nanoparticles into rat trachea. Bronchoalveolar lavage fluid (BALF) was collected after intratracheal instillation at different time points, and concentrations of lipid peroxide heme oxygenase-1 (HO-1), surfactant protein-D (SP-D) and lactate dehydrogenase (LDH) in BALF were measured.
RESULTS:The levels of intracellular reactive oxygen species and lipid peroxidation in A549 cells increased with increasing exposure to NiO nanoparticles, and increases in gene expressions of HO-1 and SP-D were observed in A549 cells. The lipid peroxide level in BALF significantly increased after 24 h instillation but decreased three days later. LDH leakage was also observed three days later.
CONCLUSIONS:NiO nanoparticles induce oxidative stress-related lung injury. In vivo and in vitro oxidative stress was induced resulting in activation of antioxidant systems. Based on these responses, we conclude that the results of the in vivo and in vitro studies tend to correspond.
巻・号 53(2)
ページ 64-74
公開日 2011-1-1
DOI 10.1539/joh.l10121
PII JST.JSTAGE/joh/L10121
PMID 21233593
MeSH Acute Disease Animals Bronchoalveolar Lavage Fluid / chemistry Gene Expression Regulation / drug effects Heme Oxygenase-1 / genetics Heme Oxygenase-1 / metabolism Humans In Vitro Techniques L-Lactate Dehydrogenase / metabolism Lipid Peroxidation / drug effects* Lung / drug effects Nanoparticles Nickel / toxicity* Oxidative Stress / drug effects* Pulmonary Surfactant-Associated Protein D / genetics Pulmonary Surfactant-Associated Protein D / metabolism Rats Rats, Wistar Reactive Oxygen Species / metabolism Tumor Cells, Cultured
IF 2.289
引用数 74
WOS 分野 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
リソース情報
ヒト・動物細胞