論文 - 詳細
| RRC ID | 38632 |
|---|---|
| 著者 | Takai T, Ohmori H. |
| タイトル | DNA transfection of mouse lymphoid cells by the combination of DEAE-dextran-mediated DNA uptake and osmotic shock procedure. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
Several mouse lymphoid cell lines were efficiently transfected with plasmid DNA by a novel method combining DEAE-dextran-mediated DNA uptake and osmotic shock procedure. The cells were first incubated with DNA-DEAE-dextran complex, treated with hypertonic Tris-HCl buffer containing 0.5 M sucrose and 10% poly(ethylene glycol), and then exposed to hypotonic RPMI 1640 medium. This transfection protocol exhibited maximal frequencies of 0.3% and 3.10(-5) for transient gene expression and stable transformation in P3-NSI/1-Ag4-1 cells, respectively. |
| 巻・号 | 1048(1) |
| ページ | 105-9 |
| 公開日 | 1990-1-30 |
| DOI | 10.1016/0167-4781(90)90029-2 |
| PII | 0167-4781(90)90029-2 |
| PMID | 2297528 |
| MeSH | Animals Cell Line DEAE-Dextran DNA / genetics* Electrophoresis, Agar Gel Gene Expression Mice Osmotic Pressure Transfection* |
| IF | 3.411 |
| 引用数 | 69 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | P3/NSI/1-AG4-1(RCB0095) |