| RRC ID |
38637
|
| Author |
Sato T, Hong MH, Jin CH, Ishimi Y, Udagawa N, Shinki T, Abe E, Suda T.
|
| Title |
The specific production of the third component of complement by osteoblastic cells treated with 1 alpha,25-dihydroxyvitamin D3.
|
| Journal |
FEBS Lett
|
| Abstract |
A 190 kDa protein was purified from conditioned media of mouse marrow-derived stromal cell (ST2) cultures treated with 1 alpha,25-dihydroxyvitamin D3 (1 alpha,25(OH)2D3) and identified as the third component of mouse complement (C3). Northern and Western blot analysis revealed that the production of C3 by ST2 and primary osteoblastic cells was strictly dependent on 1 alpha,25(OH)2D3, but the production by hepatocytes was not. Adding 1 alpha,25(OH)2D3 together with mouse C3 antibody to bone marrow cultures greatly inhibited the formation of tartrate-resistant acid phosphatase (TRAP)-positive osteoclast-like multinucleated cells. Adding C3 alone induced no TRAP-positive cell formation. These results suggest that, in bone tissues, C3 is specifically produced by osteoblasts in response to 1 alpha,25(OH)2D3 and somehow involved in inducing differentiation of bone marrow cells into osteoclasts in concert with other factors produced by osteoblasts in response to 1 alpha,25(OH)2D3.
|
| Volume |
285(1)
|
| Pages |
21-4
|
| Published |
1991-7-8
|
| DOI |
10.1016/0014-5793(91)80715-f
|
| PII |
0014-5793(91)80715-F
|
| PMID |
2065778
|
| MeSH |
Acid Phosphatase / biosynthesis
Amino Acid Sequence
Animals
Blotting, Northern
Blotting, Western
Bone Marrow Cells
Calcitriol / pharmacology*
Cell Differentiation / drug effects
Cell Line
Cells, Cultured
Complement C3 / biosynthesis*
Complement C3 / immunology
Electrophoresis, Polyacrylamide Gel
Liver / cytology
Mice
Molecular Sequence Data
Osteoblasts / drug effects
Osteoblasts / immunology
Osteoblasts / metabolism*
|
| IF |
3.057
|
| Times Cited |
41
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224) |
