| RRC ID |
38641
|
| Author |
Shioi A, Teitelbaum SL, Ross FP, Welgus HG, Suzuki H, Ohara J, Lacey DL.
|
| Title |
Interleukin 4 inhibits murine osteoclast formation in vitro.
|
| Journal |
J Cell Biochem
|
| Abstract |
Interleukin 4 (IL-4) is a product of activated T cells and mast cells with effects on immunologic and hematopoietic processes. We now report that IL-4 inhibits the formation of osteoclasts from murine bone marrow cells cocultured with stromal cells. Numerous (3,000-4,000 cells/2 cm2) tartrate-resistant acid-phosphatase-positive multinucleated cells with the capacity to generate cAMP in response to salmon calcitonin (ED50 = 10(-10) M) developed within 10-12 days of culture. IL-4 (ID50 = 10 U/ml) inhibited osteoclast generation in doses similar to those that induce proliferation of IL-4-responsive T cells. Additionally, the rat antimurine IL-4 monoclonal antibody 11B11 antagonizes the IL-4-inhibitory effect on osteoclast formation. These results suggest that IL-4 impedes agonist-induced in vitro bone resorption by inhibiting osteoclastogenesis.
|
| Volume |
47(3)
|
| Pages |
272-7
|
| Published |
1991-11-1
|
| DOI |
10.1002/jcb.240470313
|
| PMID |
1665152
|
| MeSH |
Animals
Antibodies, Monoclonal
Biomarkers
Bone Marrow / drug effects*
Bone Marrow Cells
Bone Remodeling / physiology
Calcitonin / pharmacology
Cell Differentiation / drug effects
Cell Division
Cells, Cultured
Cyclic AMP / metabolism
Interleukin-4 / immunology
Interleukin-4 / pharmacology*
Lymphocyte Activation / physiology
Mice
Osteoclasts / drug effects*
T-Lymphocytes / cytology
T-Lymphocytes / drug effects
|
| IF |
4.237
|
| Times Cited |
74
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
ST2(RCB0224) |
