RRC ID 38661
Author Takai T, Ohmori H.
Title Enhancement of DNA transfection efficiency by heat treatment of cultured mammalian cells.
Journal Biochim Biophys Acta
Abstract The expression of genes introduced into various mammalian cell lines was enhanced by raising the temperature of the cells to 42 degrees C for a few hours after DNA transfection. This heat treatment resulted in an up to 10-fold increase in the frequency of the cells that transiently expressed a foreign gene such as that of beta-galactosidase, whereas it had only a limited enhancing effect on the development of stable transformants. By immunotitration analysis, it was confirmed that the enhanced expression of beta-galactosidase activity correlated well with the increase of the enzyme protein. This procedure may have an applicability for augmenting the frequency of transient gene expression in many cell types.
Volume 1129(2)
Pages 161-5
Published 1992-1-6
DOI 10.1016/0167-4781(92)90481-e
PII 0167-4781(92)90481-E
PMID 1730054
MeSH Animals Cell Line Escherichia coli / enzymology Escherichia coli / genetics Gene Expression Genetic Vectors Hot Temperature* Immunochemistry / methods Plasmids Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Transfection* beta-Galactosidase / biosynthesis beta-Galactosidase / genetics
IF 3.411
Times Cited 14
Human and Animal Cells P3/NSI/1-AG4-1(RCB0095)