RRC ID |
38684
|
Author |
Kobayashi H, Mizuki T, Tsutsui M, Minami K, Yanagihara N, Yuhi T, Izumi F.
|
Title |
Receptors for C-type natriuretic peptide in cultured rat glial cells.
|
Journal |
Brain Res
|
Abstract |
To characterize sites of action of C-type natriuretic peptide (CNP) in the glial cells, the effect of CNP on cGMP accumulation and the binding of [125I]CNP in rat astrocyte RCR-1 cells were studied. CNP stimulated cGMP accumulation in the cells from 10(-9) M in a dose-dependent manner, but ANP (atrial natriuretic peptide) had a negligible effect on cGMP accumulation in the cells. [125I]CNP was bound to the cells and its Kd value was 2 orders of magnitude lower than that of the ED50 value for stimulation of cGMP accumulation in the cells. Not only CNP but also ANP displaced [125I]CNP binding to the cells. These results suggest that RCR-1 cells have a B-receptor which contains a guanylate cyclase domain and is preferentially activated by CNP, and that they also have a C-receptor which does not contain a guanylate cyclase domain that reacts with both ANP and CNP.
|
Volume |
617(1)
|
Pages |
163-6
|
Published |
1993-7-16
|
DOI |
10.1016/0006-8993(93)90630-6
|
PII |
0006-8993(93)90630-6
|
PMID |
8104082
|
MeSH |
Animals
Astrocytes / chemistry*
Astrocytes / metabolism
Atrial Natriuretic Factor / pharmacology
Cell Line
Cyclic GMP / metabolism
Guanylate Cyclase / analysis*
Rats
Receptors, Atrial Natriuretic Factor / analysis*
|
IF |
2.733
|
Times Cited |
19
|
WOS Category
|
NEUROSCIENCES
|
Resource |
Human and Animal Cells |
RCR-1(RCB0075) |