論文 - 詳細
| RRC ID | 38692 |
|---|---|
| 著者 | Yonei S, Tamura K, Koyama E, Nohno T, Noji S, Ide H. |
| タイトル | MRC-5, human embryonic lung fibroblasts, induce the duplication of the developing chick limb bud. |
| ジャーナル | Dev Biol |
| Abstract |
The anteroposterior (A-P) axis pattern of the chick limb is likely to be controlled by a small region of mesenchyme cells at the posterior margin of the limb bud (ZPA, zone of polarizing activity). In this study, we found that MRC-5 fibroblast cells had the capacity for duplicated-pattern formation of the chick limb along the A-P axis when grafted to the anterior region of the limb bud. MRC-5 cells were effective only during pre-limb bud stages, and the leg bud was more responsive than the wing bud. Grafted cells remained at the base of the limb bud when limb development proceeded. These results suggest that the products of MRC-5 cells are involved in three possible processes of duplicated-pattern formation: induction of the polarizing activity; maintenance of this activity, which is present weakly at pre-limb bud stages; and determination of A-P axis as the ZPA factor(s). By allowing the use of a small number of cells of embryonic tissues with polarizing activity, the analysis of duplicate formation with the MRC-5 cell line provides a powerful tool for elucidating the molecular nature of the ZPA. |
| 巻・号 | 160(1) |
| ページ | 246-53 |
| 公開日 | 1993-11-1 |
| DOI | 10.1006/dbio.1993.1302 |
| PII | S0012-1606(83)71302-3 |
| PMID | 8224541 |
| MeSH | 3T3 Cells Animals Biological Factors / biosynthesis Cell Polarity Chick Embryo Embryonic Induction Extremities / embryology* Extremities / growth & development Fibroblasts / metabolism Fibroblasts / physiology* Fibroblasts / transplantation Humans Lung / cytology Lung / embryology* Mesoderm / cytology Mice Wings, Animal / embryology |
| IF | 2.896 |
| 引用数 | 8 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MRC-5(RCB0211) MRC-5(RCB0218) |