論文 - 詳細
| RRC ID | 38715 |
|---|---|
| 著者 | Umayahara Y, Kawamori R, Watada H, Imano E, Iwama N, Morishima T, Yamasaki Y, Kajimoto Y, Kamada T. |
| タイトル | Estrogen regulation of the insulin-like growth factor I gene transcription involves an AP-1 enhancer. |
| ジャーナル | J Biol Chem |
| Abstract |
As a step toward elucidating the physiological role of insulin-like growth factor-I (IGF-I) in mediating estrogen action, we sought to determine the molecular basis of the phenomenon. In HepG2 cells expressing exogenous estrogen receptors (ER), a reporter gene plasmid containing 600 base pairs of the chicken IGF-I promoter enhanced expression of luciferase 8.6-fold in response to 10(-6) M 17 beta-estradiol, indicating that the IGF-I promoter is a target of estrogen regulation. Although no conventional estrogen-responsive element was identified within the promoter fragment, the AP-1 motif located therein was shown to be essential; the estrogen-responsive enhancement of the Fos-Jun binding to the AP-1 motif, which takes place by means of post-translational modification, mediates the estrogen action. A direct or indirect interaction between the estrogen-ER complex and the Fos-Jun complex seems to facilitate the Fos-Jun binding to the target DNA. Although ER binding to the target DNA was not considered to be involved in the signaling pathway, the DNA binding domain-deficient ER did not mediate the phenomenon, providing support for the existence of a unique function of the DNA binding domain of ER in facilitating some protein-protein interaction. In conclusion, our present observations demonstrate that the chicken IGF-I gene promoter is controlled by estrogen through a unique pathway involving Fos, Jun, and the DNA binding domain of ER. |
| 巻・号 | 269(23) |
| ページ | 16433-42 |
| 公開日 | 1994-6-10 |
| PII | S0021-9258(17)34025-5 |
| PMID | 8206951 |
| MeSH | Animals Base Sequence Cells, Cultured Chickens DNA Mutational Analysis Enhancer Elements, Genetic / genetics* Estradiol / pharmacology* Gene Expression Regulation / drug effects* Gene Transfer Techniques Genes, Reporter Humans Insulin-Like Growth Factor I / biosynthesis* Insulin-Like Growth Factor I / genetics Luciferases / biosynthesis Luciferases / genetics Molecular Sequence Data Promoter Regions, Genetic / genetics Protein Binding Protein Processing, Post-Translational Proto-Oncogene Proteins c-fos / metabolism Proto-Oncogene Proteins c-jun / metabolism* Receptors, Estrogen / metabolism Signal Transduction Transcription, Genetic* |
| IF | 4.238 |
| 引用数 | 308 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0459) |