RRC ID 3872
Author Ando M, Tu W, Nishijima K, Iijima S.
Title Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs.
Journal Biochem Biophys Res Commun
Abstract We examined whether Siglec-9 modulates cytokine production in the macrophage cell line RAW264. Cells expressing Siglec-9 produced low levels of tumor necrosis factor (TNF)-alpha upon stimulation with lipopolysaccharide, peptidoglycan, unmethylated CpG DNA, and double-stranded RNA. On the other hand, interleukin (IL)-10 production was strongly enhanced in Siglec-9-expressing cells. Similar activities were also exhibited by Siglec-5. However, the up-regulation of IL-10 as well as the down-regulation of TNF-alpha was abrogated when two tyrosine residues in the cytoplasmic tail of Siglec-9 were mutated to phenylalanine. A membrane proximal ITIM mutant of Siglec-9 did not enhance IL-10 production but partly inhibited TNF-alpha production, indicating diverse regulation mechanisms of TNF-alpha and IL-10. Siglec-9 also enhanced the production of IL-10 in the human macrophage cell line THP-1. These results demonstrate that Siglec-9 enhances the production of the anti-inflammatory cytokine IL-10 in macrophages.
Volume 369(3)
Pages 878-83
Published 2008-5-9
DOI 10.1016/j.bbrc.2008.02.111
PII S0006-291X(08)00390-2
PMID 18325328
MeSH Amino Acid Motifs Animals Antigens, CD / genetics Antigens, CD / metabolism* Antigens, Differentiation, Myelomonocytic / genetics Antigens, Differentiation, Myelomonocytic / metabolism CpG Islands / immunology Humans Interleukin-10 / metabolism* Lectins / genetics Lectins / metabolism* Lipopolysaccharides / immunology Lipopolysaccharides / pharmacology Macrophages / drug effects Macrophages / immunology* Mice Peptidoglycan / immunology Peptidoglycan / pharmacology Poly I-C / immunology Poly I-C / pharmacology Sialic Acid Binding Immunoglobulin-like Lectins Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / metabolism Tyrosine / chemistry Tyrosine / genetics
IF 2.705
Times Cited 67
Human and Animal Cells