Reference - Detail
| RRC ID | 3872 |
|---|---|
| Author | Ando M, Tu W, Nishijima K, Iijima S. |
| Title | Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
We examined whether Siglec-9 modulates cytokine production in the macrophage cell line RAW264. Cells expressing Siglec-9 produced low levels of tumor necrosis factor (TNF)-alpha upon stimulation with lipopolysaccharide, peptidoglycan, unmethylated CpG DNA, and double-stranded RNA. On the other hand, interleukin (IL)-10 production was strongly enhanced in Siglec-9-expressing cells. Similar activities were also exhibited by Siglec-5. However, the up-regulation of IL-10 as well as the down-regulation of TNF-alpha was abrogated when two tyrosine residues in the cytoplasmic tail of Siglec-9 were mutated to phenylalanine. A membrane proximal ITIM mutant of Siglec-9 did not enhance IL-10 production but partly inhibited TNF-alpha production, indicating diverse regulation mechanisms of TNF-alpha and IL-10. Siglec-9 also enhanced the production of IL-10 in the human macrophage cell line THP-1. These results demonstrate that Siglec-9 enhances the production of the anti-inflammatory cytokine IL-10 in macrophages. |
| Volume | 369(3) |
| Pages | 878-83 |
| Published | 2008-5-9 |
| DOI | 10.1016/j.bbrc.2008.02.111 |
| PII | S0006-291X(08)00390-2 |
| PMID | 18325328 |
| MeSH | Amino Acid Motifs Animals Antigens, CD / genetics Antigens, CD / metabolism* Antigens, Differentiation, Myelomonocytic / genetics Antigens, Differentiation, Myelomonocytic / metabolism CpG Islands / immunology Humans Interleukin-10 / metabolism* Lectins / genetics Lectins / metabolism* Lipopolysaccharides / immunology Lipopolysaccharides / pharmacology Macrophages / drug effects Macrophages / immunology* Mice Peptidoglycan / immunology Peptidoglycan / pharmacology Poly I-C / immunology Poly I-C / pharmacology Sialic Acid Binding Immunoglobulin-like Lectins Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / metabolism Tyrosine / chemistry Tyrosine / genetics |
| IF | 2.985 |
| Times Cited | 67 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 12 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |