| RRC ID |
3872
|
| 著者 |
Ando M, Tu W, Nishijima K, Iijima S.
|
| タイトル |
Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We examined whether Siglec-9 modulates cytokine production in the macrophage cell line RAW264. Cells expressing Siglec-9 produced low levels of tumor necrosis factor (TNF)-alpha upon stimulation with lipopolysaccharide, peptidoglycan, unmethylated CpG DNA, and double-stranded RNA. On the other hand, interleukin (IL)-10 production was strongly enhanced in Siglec-9-expressing cells. Similar activities were also exhibited by Siglec-5. However, the up-regulation of IL-10 as well as the down-regulation of TNF-alpha was abrogated when two tyrosine residues in the cytoplasmic tail of Siglec-9 were mutated to phenylalanine. A membrane proximal ITIM mutant of Siglec-9 did not enhance IL-10 production but partly inhibited TNF-alpha production, indicating diverse regulation mechanisms of TNF-alpha and IL-10. Siglec-9 also enhanced the production of IL-10 in the human macrophage cell line THP-1. These results demonstrate that Siglec-9 enhances the production of the anti-inflammatory cytokine IL-10 in macrophages.
|
| 巻・号 |
369(3)
|
| ページ |
878-83
|
| 公開日 |
2008-5-9
|
| DOI |
10.1016/j.bbrc.2008.02.111
|
| PII |
S0006-291X(08)00390-2
|
| PMID |
18325328
|
| MeSH |
Amino Acid Motifs
Animals
Antigens, CD / genetics
Antigens, CD / metabolism*
Antigens, Differentiation, Myelomonocytic / genetics
Antigens, Differentiation, Myelomonocytic / metabolism
CpG Islands / immunology
Humans
Interleukin-10 / metabolism*
Lectins / genetics
Lectins / metabolism*
Lipopolysaccharides / immunology
Lipopolysaccharides / pharmacology
Macrophages / drug effects
Macrophages / immunology*
Mice
Peptidoglycan / immunology
Peptidoglycan / pharmacology
Poly I-C / immunology
Poly I-C / pharmacology
Sialic Acid Binding Immunoglobulin-like Lectins
Tumor Necrosis Factor-alpha / antagonists & inhibitors
Tumor Necrosis Factor-alpha / metabolism
Tyrosine / chemistry
Tyrosine / genetics
|
| IF |
2.985
|
| 引用数 |
67
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
