| RRC ID |
38735
|
| Author |
Kondo S, Yin D, Takeuchi J, Morimura T, Miyatake SI, Nakatsu S, Oda Y, Kikuchi H.
|
| Title |
Tumour necrosis factor-alpha induces an increase in susceptibility of human glioblastoma U87-MG cells to natural killer cell-mediated lysis.
|
| Journal |
Br J Cancer
|
| Abstract |
The mechanism by which tumour necrosis factor (TNF)-alpha increases the susceptibility of U87-MG human glioblastoma cells to lysis by natural killer (NK) cells was studied. Treatment with TNF-alpha (100 units ml-1) for 48 h enhanced the susceptibility of tumour cells to lysis by NK cells. Increased susceptibility to lysis was associated with enhanced expression of intercellular adhesion molecule 1 (ICAM-1) and HLA class I antigen. Antisense ICAM-1 oligonucleotide inhibited lysis by NK cells of TNF-alpha-treated tumour cells. In contrast, acid treatment following TNF-alpha treatment increased lysis by NK cells. These findings indicate that TNF-alpha treatment of glioblastoma cells increased their susceptibility to lysis by NK cells, since ICAM-1 up-regulation would have more profound effects on NK susceptibility than would HLA class I antigen up-regulation.
|
| Volume |
69(4)
|
| Pages |
627-32
|
| Published |
1994-4-1
|
| DOI |
10.1038/bjc.1994.123
|
| PMID |
7908214
|
| PMC |
PMC1968817
|
| MeSH |
Antigens, Neoplasm / biosynthesis
Antigens, Neoplasm / immunology
Base Sequence
Cell Adhesion Molecules / biosynthesis
Cell Adhesion Molecules / immunology
Cytotoxicity, Immunologic / drug effects*
Fluorescent Antibody Technique
Glioblastoma / immunology*
Glioblastoma / pathology
Histocompatibility Antigens Class I / biosynthesis
Histocompatibility Antigens Class I / immunology
Humans
Intercellular Adhesion Molecule-1
Killer Cells, Natural / physiology*
Molecular Sequence Data
Oligonucleotides, Antisense / metabolism
Tumor Cells, Cultured / drug effects
Tumor Cells, Cultured / immunology
Tumor Necrosis Factor-alpha / pharmacology*
Up-Regulation
|
| IF |
5.791
|
| Times Cited |
19
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| Human and Animal Cells |
U-87 MG(RCB0419) |
