RRC ID |
38739
|
著者 |
Yin D, Kondo S, Takeuchi J, Morimura T, Nakatsu S, Oda Y, Kikuchi H.
|
タイトル |
Interferon-gamma induces a decrease in the susceptibility of human glioma cells to lysis by lymphokine-activated killer cells.
|
ジャーナル |
Neurosurgery
|
Abstract |
We studied the effect that treating two types of glioblastoma cell lines, U-87 MG and U-251 MG, with interferon (IFN)-gamma had on their susceptibility to lysis by lymphokine-activated killer (LAK) cells. We also examined the participation of cell-adhesion molecules and major histocompatibility complex (MHC) class I and II antigens present on the target cells in lysis by LAK cells. Treatment with IFN-gamma (1000 U/ml) for 48 hours resulted in the increased expression of both intercellular-adhesion molecule 1 and MHC class I antigens on tumor cells. In addition, untreated tumor cells expressed neural-cell-adhesion molecules and MHC class II antigens highly, but their expression was not affected by IFN-gamma treatment. These changes in expression were accompanied by a decreased susceptibility to lysis by LAK cells. Treatment with antisense-intercellular-adhesion molecule-1 oligonucleotide further inhibited LAK lysis of target cells, following treatment with IFN-gamma. In contrast, acid treatment of tumor cells after treatment with IFN-gamma increased their susceptibility to lysis by LAK cells. These findings suggest that treatment of glioblastoma cells with IFN-gamma decreased their susceptibility to lysis by LAK cells, and that this decrease in susceptibility is attributable principally to the increased expression of MHC class I antigen on target cells.
|
巻・号 |
35(1)
|
ページ |
113-8
|
公開日 |
1994-7-1
|
DOI |
10.1227/00006123-199407000-00017
|
PMID |
7936131
|
MeSH |
Base Sequence
Cell Adhesion Molecules, Neuronal / analysis
Cell Line
Glioblastoma / immunology*
Histocompatibility Antigens Class I / analysis
Histocompatibility Antigens Class II / analysis
Humans
Intercellular Adhesion Molecule-1 / analysis
Interferon-gamma / pharmacology*
Killer Cells, Lymphokine-Activated / physiology*
Molecular Sequence Data
Tumor Cells, Cultured
|
IF |
4.853
|
引用数 |
5
|
WOS 分野
|
SURGERY
CLINICAL NEUROLOGY
|
リソース情報 |
ヒト・動物細胞 |
U-87 MG(RCB0419)
U251(RCB0461) |