RRC ID 38739
著者 Yin D, Kondo S, Takeuchi J, Morimura T, Nakatsu S, Oda Y, Kikuchi H.
タイトル Interferon-gamma induces a decrease in the susceptibility of human glioma cells to lysis by lymphokine-activated killer cells.
ジャーナル Neurosurgery
Abstract We studied the effect that treating two types of glioblastoma cell lines, U-87 MG and U-251 MG, with interferon (IFN)-gamma had on their susceptibility to lysis by lymphokine-activated killer (LAK) cells. We also examined the participation of cell-adhesion molecules and major histocompatibility complex (MHC) class I and II antigens present on the target cells in lysis by LAK cells. Treatment with IFN-gamma (1000 U/ml) for 48 hours resulted in the increased expression of both intercellular-adhesion molecule 1 and MHC class I antigens on tumor cells. In addition, untreated tumor cells expressed neural-cell-adhesion molecules and MHC class II antigens highly, but their expression was not affected by IFN-gamma treatment. These changes in expression were accompanied by a decreased susceptibility to lysis by LAK cells. Treatment with antisense-intercellular-adhesion molecule-1 oligonucleotide further inhibited LAK lysis of target cells, following treatment with IFN-gamma. In contrast, acid treatment of tumor cells after treatment with IFN-gamma increased their susceptibility to lysis by LAK cells. These findings suggest that treatment of glioblastoma cells with IFN-gamma decreased their susceptibility to lysis by LAK cells, and that this decrease in susceptibility is attributable principally to the increased expression of MHC class I antigen on target cells.
巻・号 35(1)
ページ 113-8
公開日 1994-7-1
DOI 10.1227/00006123-199407000-00017
PMID 7936131
MeSH Base Sequence Cell Adhesion Molecules, Neuronal / analysis Cell Line Glioblastoma / immunology* Histocompatibility Antigens Class I / analysis Histocompatibility Antigens Class II / analysis Humans Intercellular Adhesion Molecule-1 / analysis Interferon-gamma / pharmacology* Killer Cells, Lymphokine-Activated / physiology* Molecular Sequence Data Tumor Cells, Cultured
IF 4.853
引用数 5
WOS 分野 SURGERY CLINICAL NEUROLOGY
リソース情報
ヒト・動物細胞 U-87 MG(RCB0419) U251(RCB0461)