| RRC ID |
38747
|
| Author |
Adachi Y, Okazaki M, Ohno N, Yadomae T.
|
| Title |
Enhancement of cytokine production by macrophages stimulated with (1-->3)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa.
|
| Journal |
Biol Pharm Bull
|
| Abstract |
The ability of grifolan (GRN), a purified fungal (1-->3)-beta-D-glucan, to induce various cytokines from macrophages was examined in vitro. Interleukin-6 (IL-6) activity in supernatants from the culture of macrophage cell line, RAW264.7 was dependent on increasing doses of GRN. The level of IL-6 induced with 500 micrograms/ml of GRN was comparable to that induced with lipopolysaccharide (LPS) 10 micrograms/ml. Enhancement of the mRNA level of IL-6 by treatment with GRN was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). The effect of GRN on production of IL-6 was also observed using peritoneal macrophages from C3H/HeJ mice which did not respond to endotoxins. This data suggested that the ability of GRN to activate IL-6 production of macrophages is not due to contamination of endotoxins in the preparation. Enhanced production of cytokine by GRN was observed not only with IL-6, but also with interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF alpha). In the production of TNF alpha, GRN was more effective than LPS used in this study. Other soluble or gel-forming(1-->3)-beta-D-glucans from various sources did not enhance the production of such cytokines although they are structurally similar to GRN. The above results indicate that GRN is a novel macrophage activator which augments cytokine production without dependence on endotoxins.
|
| Volume |
17(12)
|
| Pages |
1554-60
|
| Published |
1994-12-1
|
| DOI |
10.1248/bpb.17.1554
|
| PMID |
7537572
|
| MeSH |
Adjuvants, Immunologic / isolation & purification
Adjuvants, Immunologic / pharmacology*
Animals
Base Sequence
Cytokines / biosynthesis*
Glucans / isolation & purification
Glucans / pharmacology
In Vitro Techniques
Interleukin-1 / biosynthesis
Interleukin-6 / biosynthesis
Lipopolysaccharides / pharmacology
Macrophages, Peritoneal / drug effects
Macrophages, Peritoneal / metabolism*
Mice
Mice, Inbred C3H
Molecular Sequence Data
Plants, Medicinal / chemistry*
Polymerase Chain Reaction
RNA-Directed DNA Polymerase
T-Lymphocytes / drug effects
Tumor Necrosis Factor-alpha / biosynthesis
beta-Glucans*
|
| IF |
1.863
|
| Times Cited |
159
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
