論文 - 詳細
| RRC ID | 38750 |
|---|---|
| 著者 | Nishihara T, Akifusa S, Koseki T, Kato S, Muro M, Hanada N. |
| タイトル | Specific inhibitors of vacuolar type H(+)-ATPases induce apoptotic cell death. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Concanamycin A and bafilomycin A1 are known as strong inhibitors of the vacuolar type H(+)-ATPases in vitro. These inhibitors exhibited cytotoxic effects on twelve cell lines in cell viability assay. On the other hand, the F1F0-type H(+)-ATPase inhibitor oligomycin and the E1E2-type H(+)-ATPase inhibitor vanadate showed no cytotoxic effect. We show here that concanamycin A and bafilomycin A1 induce a significant increase in the proportion of fragmented DNA in agarose gel electrophoresis. Flow cytometric cell cycle analysis of WEHI 231 cells stimulated with concanamycin A revealed the increased percentage of apoptotic cells with hypodiploid DNA. These findings indicate that cell death induced by specific inhibitors of vacuolar type H(+)-ATPases occurs through apoptosis. |
| 巻・号 | 212(1) |
| ページ | 255-62 |
| 公開日 | 1995-7-6 |
| DOI | 10.1006/bbrc.1995.1964 |
| PII | S0006-291X(85)71964-X |
| PMID | 7612014 |
| MeSH | Animals Anti-Bacterial Agents / pharmacology* Apoptosis / drug effects* Cell Line Cell Survival / drug effects DNA, Neoplasm / drug effects DNA, Neoplasm / isolation & purification Dose-Response Relationship, Drug Flow Cytometry Humans Macrolides* Mice Proton-Translocating ATPases / antagonists & inhibitors* Tumor Cells, Cultured Vacuoles / enzymology* |
| IF | 2.985 |
| 引用数 | 63 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0459) |