論文 - 詳細
| RRC ID | 38761 |
|---|---|
| 著者 | Kato G, Maeda S. |
| タイトル | Novel phosphorylation at a mitotic site, serine 75, in human pp60c-src from unsynchronized human tumor cells having a spherical morphology. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Endogenous pp60c-src from Y79 unsynchronized human retinoblastoma cells is phosphorylated at an additional N-terminal serine residue relative to unsynchronized fibroblast pp60c-src. We confirmed that the novel phosphorylation site is Ser 75, which is the same as that phosphorylated in overexpressed human pp60c-src during NIH3T3 cell mitosis. We also showed that the Ser 75 phosphorylation pattern correlates with cell rounding in 14 various unsynchronized human tumor cell lines. Especially, pp60c-src from Lu135 having a spherical morphology similar to that of Y79 is phosphorylated as high as Y79 pp60c-src. Mitotic spherical cells of the epithelial-like HepG2 express pp60c-src phosphorylated on Ser 75. On the other hand, Y79 pp60c-src is phosphorylated on Ser 75 throughout the cell cycle. These data suggest that this phosphorylation may be important in spherical cell morphology. |
| 巻・号 | 216(2) |
| ページ | 619-29 |
| 公開日 | 1995-11-13 |
| DOI | 10.1006/bbrc.1995.2667 |
| PII | S0006-291X(85)72667-8 |
| PMID | 7488156 |
| MeSH | 3T3 Cells Amino Acid Sequence Animals Cell Adhesion Cell Cycle / drug effects Cell Line Eye Neoplasms Fibroblasts / metabolism Humans Mice Mitosis* / drug effects Molecular Sequence Data Nocodazole / pharmacology Peptide Fragments / chemistry Peptide Fragments / isolation & purification Phosphates / metabolism Phosphorylation Proto-Oncogene Proteins pp60(c-src) / chemistry* Proto-Oncogene Proteins pp60(c-src) / metabolism* Recombinant Proteins / chemistry Recombinant Proteins / metabolism Retinoblastoma Serine* Transfection Tumor Cells, Cultured |
| IF | 2.985 |
| 引用数 | 6 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | Saos-2(RCB0428) T24(RCB0431) Hep G2(RCB0459) A431(RCB0202) Lu-130(RCB0465) Lu-135(RCB0468) Lu-140(RCB0470) |