Reference - Detail
| RRC ID | 38762 |
|---|---|
| Author | Ishizuka T, Ino H, Sawa K, Suzuki N, Tatibana M. |
| Title | Promoter region of the mouse cyclin-dependent kinase 5-encoding gene. |
| Journal | Gene |
| Abstract |
While cyclin-dependent kinases, such as CDC2 and CDK2, are key regulators of cell-cycle progression, cyclin-dependent kinase 5 (CDK5) is highly expressed in mature neurons with no evident cell-cycle regulation. The 5'-region of the mouse CDK5 gene was isolated and sequenced. The isolated clone included exons 1 through 7. The 5'-flanking region has a high G+C content. There is no TATA box around the transcriptional start points (tsp), as determined by primer extension analysis. One CCAAT box, one AP-1-binding site, two AP-2-binding sites, and one cAMP-responsive element are located upstream from the tsp. Promoter/cat fusion assays showed that the 5.8-kb fragment of this 5'-flanking sequence possessed the promoter activities expressing cat in rat PC12 pheochromocytoma cells. The effect of deletions of the promoter suggested the presence of two negative control elements located from -2.9 kb to -546 bp, and from -212 to -155 upstream from the 5' end of the tsp. Two positive elements from bp -300 to -212 and from -155 to -41 were also detected. In the element from bp -300 to -212, there was a putative NF-IL6-binding sequence. Thus, the CDK5 promoter region contains multiple positive and negative cis-acting regulatory elements, an arrangement which suggests that the regulation of transcription of CDK5 is under complex control. |
| Volume | 166(2) |
| Pages | 267-71 |
| Published | 1995-12-12 |
| DOI | 10.1016/0378-1119(95)00650-8 |
| PII | 0378111995006508 |
| PMID | 8543173 |
| MeSH | Animals Base Sequence Cyclin-Dependent Kinase 5 Cyclin-Dependent Kinases* Exons Gene Expression Regulation, Enzymologic Male Mice Molecular Sequence Data Neurons / enzymology Promoter Regions, Genetic* Protein Serine-Threonine Kinases / genetics* RNA, Messenger / genetics Restriction Mapping Transcription, Genetic |
| IF | 2.984 |
| Times Cited | 14 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | PC-12(RCB0009) |