Reference - Detail
| RRC ID | 38767 |
|---|---|
| Author | Jimi E, Shuto T, Koga T. |
| Title | Macrophage colony-stimulating factor and interleukin-1 alpha maintain the survival of osteoclast-like cells. |
| Journal | Endocrinology |
| Abstract |
When mouse bone marrow cells were co-cultured with a stromal cell line. ST2, numerous osteoclast-like multinucleated cells (MNCs) were formed. To enrich the MNCs which were tartrate-resistant acid phosphatase (TRAP)-positive, we treated the cultures with dispase. Enriched TRAP-positive MNCs adhering to the bottom of the dish were cultured for a further 24h with medium containing 15% fetal calf serum. More than 80% of TRAP-positive MNCs were detached from the dish during the culture. However, when ST2 cells were included during the culture period, survival of TRAP-positive MNCs were significantly increased. Moreover, among various factors examined for the survival of TRAP-positive MNCs, only M-CSF and IL-1 alpha were effective in prolonging viability of TRAP-positive MNCs. |
| Volume | 136(2) |
| Pages | 808-11 |
| Published | 1995-2-1 |
| DOI | 10.1210/endo.136.2.7835314 |
| PMID | 7835314 |
| MeSH | Acid Phosphatase Animals Bone Marrow / metabolism* Bone Marrow Cells Cell Survival / drug effects Cells, Cultured Interleukin-1 / pharmacology* Macrophage Colony-Stimulating Factor / pharmacology* Mice Osteoclasts / cytology* Rats Stromal Cells / cytology Tartrates |
| IF | 3.934 |
| Times Cited | 152 |
| WOS Category | ENDOCRINOLOGY & METABOLISM |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ST2(RCB0224) |