論文 - 詳細
RRC ID | 38770 |
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著者 | Fukuhara S, Mukai H, Munekata E. |
タイトル | Pharmacological evidence for neurokinin receptors in murine neuroblastoma C1300 cells. |
ジャーナル | Peptides |
Abstract |
We found that neurokinin A (NKA) and neurokinin B (NKB) induce an increase in the concentration of intracellular free Ca2+ ([Ca2+]i) in murine neuroblastoma C1300 cells (EC50: NKA 87 +/- 13 nM, NKB 97 +/- 15 nM). Substance P (SP) also caused a transient Ca2+ increase, although the potency of SP was much less than that of NKA and NKB. The increase in [Ca2+]i induced by NKA and NKB was inhibited by SR 48,968, a selective antagonist for NK2, and [beta Ala8]NKA(4-10), a selective agonist for NK2, did not stimulate the increase in [Ca2+]i. NKA- and NKB-induced Ca2+ mobilization was not inhibited by CP-96,345 and [Trp7, beta Ala8]NKA(4-10), selective antagonists for NK1 and NK3, respectively. These results suggested that C1300 cells express endogenous NK2 neurokinin receptors that have different features from known NK2 receptors. |
巻・号 | 16(2) |
ページ | 211-4 |
公開日 | 1995-1-1 |
DOI | 10.1016/0196-9781(94)00166-9 |
PII | 0196-9781(94)00166-9 |
PMID | 7540290 |
MeSH | Animals Benzamides / pharmacology Calcium / metabolism* Cell Line Kinetics Mice Neuroblastoma Neurokinin A / antagonists & inhibitors Neurokinin A / pharmacology* Neurokinin B / antagonists & inhibitors Neurokinin B / pharmacology* Piperidines / pharmacology Receptors, Tachykinin / analysis Receptors, Tachykinin / antagonists & inhibitors Receptors, Tachykinin / physiology* Substance P / pharmacology* Tumor Cells, Cultured |
IF | 2.843 |
引用数 | 11 |
WOS 分野 | PHARMACOLOGY & PHARMACY ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | C-1300(RCB0283) |